A novel recycling mechanism of native IgE-antigen complexes in human B cells facilitates transfer of antigen to dendritic cells for antigen presentation

The Journal of Allergy and Clinical Immunology
Paul EngeroffMonique Vogel

Abstract

IgE-immune complexes (IgE-ICs) have been shown to enhance antibody and T-cell responses in mice by targeting CD23 (FcεRII), the low-affinity receptor for IgE on B cells. In humans, the mechanism by which CD23-expressing cells take up IgE-ICs and process them is not well understood. To investigate this question, we compared the fate of IgE-ICs in human B cells and in CD23-expressing monocyte-derived dendritic cells (moDCs) that represent classical antigen-presenting cells and we aimed at studying IgE-dependent antigen presentation in both cell types. B cells and monocytes were isolated from peripheral blood, and monocytes were differentiated into moDCs. Both cell types were stimulated with IgE-ICs consisting of 4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP)-specific IgE JW8 and NIP-BSA to assess binding, uptake, and degradation dynamics. To assess CD23-dependent T-cell proliferation, B cells and moDCs were pulsed with IgE-NIP-tetanus toxoid complexes and cocultured with autologous T cells. IgE-IC binding was CD23-dependent in B cells, and moDCs and CD23 aggregation, as well as IgE-IC internalization, occurred in both cell types. Although IgE-ICs were degraded in moDCs, B cells did not degrade the complexes but recycled them in nativ...Continue Reading

Citations

Dec 4, 2019·Current Opinion in Allergy and Clinical Immunology·Timothy G Chow, Michelle A Gill
Aug 31, 2019·Cells·Julia Eckl-DornaRudolf Valenta
Aug 14, 2020·International Journal of Molecular Sciences·Verena K KöhlerErika Jensen-Jarolim
Dec 15, 2020·The Journal of Allergy and Clinical Immunology·Paul EngeroffMonique Vogel
Dec 31, 2020·Allergy·Paul Engeroff, Monique Vogel
Mar 24, 2021·The Journal of Allergy and Clinical Immunology. in Practice·Erika Jensen-JarolimIsabella Pali-Schöll
May 5, 2021·The Journal of Experimental Medicine·Xiaoying ZhouKari C Nadeau
May 18, 2021·Allergy·Mohamed H ShamjiR J Joost van Neerven

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