Abstract
Antibiotic resistance is a major therapeutic problem in patients infected with Helicobacter pylori. H. pylori clarithromycin resistant mutants have been evolved during antibiotic therapy, this is mainly due to 23s rRNA point mutations. In the present study, we investigated anti-mutational features of four traditionally Iranian medicinal plants on three local isolated H. pylori strains. In this study clarithromycin resistance was used as a mutation indicator. Frequencies of such mutations in the presence and absence of plant extracts were evaluated. Mutation incidence was evaluated by Luria Delbruck fluctuation assay. The mean mutation frequency in H. pylori isolates was 27 × 10(-9) which decreased at the presence of Mirtus communis, Teucrium polium, Achillea millefolium and Thymus vulgaris of plant extract, this amount was 97.4%, 95.2%, 63.7% and 19.6% respectively. Moreover, A-to-G transition at 2143 position (A2143G) was detected by PCR-sequencing as major point mutation causing clarithromycin resistant mutants. The efficacy of these plant extracts in prohibiting resistance showed considerable results. This finding should be considered to use plant extracts with antibiotics to develop more effective eradication regimens.
References
Apr 15, 1985·The Medical Journal of Australia·B J MarshallR J Glancy
Feb 1, 1996·Antimicrobial Agents and Chemotherapy·J VersalovicM F Go
Jan 7, 1998·Antimicrobial Agents and Chemotherapy·D E TaylorK Hiratsuka
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W A Rosche, P L Foster
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·G WangD E Taylor
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B BjörkholmD I Andersson
Oct 12, 2004·Mutation Research·N HayderL Chekir-Ghedira
May 5, 2005·PLoS Biology·Ryan T CirzFloyd E Romesberg
Sep 22, 2005·International Journal of Medical Microbiology : IJMM·Christian Kraft, Sebastian Suerbaum
Dec 1, 2005·Phytotherapy Research : PTR·Gail B MahadyLucas R Chadwick
Aug 8, 2006·Current Opinion in Microbiology·Dan I Andersson
Jun 15, 2007·FEMS Immunology and Medical Microbiology·Hilde de Reuse, Stefan Bereswill
Sep 5, 2009·The Journal of Clinical Investigation·John C Atherton, Martin J Blaser
Dec 17, 2009·The Journal of Antimicrobial Chemotherapy·Vincenzo De FrancescoDino Vaira
Mar 10, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Wissem Aidi WannesBrahim Marzouk
Apr 30, 2010·The New England Journal of Medicine·Kenneth E L McColl
May 27, 2010·Journal of Agricultural and Food Chemistry·Alfreda Wei, Takayuki Shibamoto
Dec 17, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Graça Miguel
Mar 22, 2012·Journal of Ethnopharmacology·Syed Faisal ZaidiToshiro Sugiyama
Apr 12, 2012·Gut·Peter MalfertheinerUNKNOWN European Helicobacter Study Group
May 15, 2012·Gut·Francis MegraudUNKNOWN Study Group participants
Jul 26, 2012·Gastroenterology Research and Practice·Wenming WuGang Sun
Jan 10, 2013·Cytotechnology·Olivera Milošević-DjordjevićDarko Grujičić