PMID: 9194531May 1, 1997Paper

A novel regulatory function of proteolytically cleaved VEGF-2 for vascular endothelial and smooth muscle cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
J S HuT A Coleman

Abstract

By high throughput sequencing, we have identified a cDNA encoding a polypeptide related to vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) in the VEGF/PDGF gene family. It is designated vascular endothelial growth factor 2 (VEGF-2). Similar to VEGF, expression of VEGF-2 mRNA is abundant in vascular smooth muscle cells and several highly vascularized tissues. VEGF-2 protein is expressed as a secreted 52 kDa precursor as well as the 30 kDa amino-terminal and 27 kDa carboxy-terminal cleavage products. The latter two cleavage products are linked via a disulfide bridge (or bridges) and can be copurified. Using copurified 30 and 27 kDa proteins, the effect of VEGF-2 on growth of several cell types, including vascular endothelial and smooth muscle cells, was determined. Our results demonstrate that VEGF-2 protein stimulates the growth of human vascular endothelial cells but inhibits growth of human aortic smooth muscle cells induced by platelet-derived growth factor. These studies establish VEGF-2 as a novel regulator for growth of vascular endothelial and smooth muscle cells.

Citations

Feb 28, 2006·Nature Clinical Practice. Cardiovascular Medicine·Satoshi ShintaniDouglas W Losordo
Jun 24, 2000·Annals of the New York Academy of Sciences·P Carmeliet, D Collen
Oct 6, 1997·The Journal of Biological Chemistry·D ChilovK Alitalo
Dec 18, 1998·Journal of Hypertension·F A le NobleH A Struijker Boudier
Apr 6, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Adam D Durbin, Avrum I Gotlieb
Jun 2, 1998·Kidney International·P Carmeliet, D Collen
Aug 26, 1998·The American Journal of Pathology·B WitzenbichlerJ M Isner

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