A novel regulatory mechanism for a novel phase-variable outer membrane protein of Escherichia coli

Advances in Experimental Medicine and Biology
I R HendersonP Owen

Abstract

Antigen 43 (Ag43) is a prominent hetero-oligomeric protein complex in the outer membrane of Escherichia coli. It is composed of two subunits. alpha 43 (M(r) 60, 000) and beta 43 (M(r) 53, 000) in 1:1 stoichiometry. alpha 43 is surface expressed, extends beyond the O-side chains of smooth lipopolysaccharide and is bound to the cell surface through an interaction with beta 43, itself an integral outer membrane protein, alpha 43 shows limited sequence homology with some enterobacterial adhesins. Expression of Ag43 is subject to reversible phase variation, the rates of variation from the Ag43+ve to Ag43-ve states in liquid minimal medium being approximately 2.2 x 10(-3), the corresponding rates from Ag43-ve to Ag43+ve states being approximately 1 x 10(-3). Phase switching of genes encoding Ag43 are transcriptionally regulated by DNA methylation (deoxyadenosine methylase [dam] mutants being "locked OFF") and by OxyR (oxyR mutants being "locked ON"). It is proposed that OxyR acts as a repressor of Ag43 transcription by binding to unmethylated GATC sites in the regulatory region of the gene. Sequencing and mapping has identified Ag43 as the likely product of the metastable flu gene first described in 1980 by Diderichsen and responsibl...Continue Reading

Citations

Sep 4, 1999·Molecular Microbiology·I R HendersonJ P Nataro
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D M HeithoffM J Mahan
Mar 20, 2003·Journal of Bacteriology·Anu WallechaMarjan van der Woude
Mar 27, 2007·Applied and Environmental Microbiology·Sandra Da ReChristophe Beloin
May 30, 2008·FEMS Microbiology Letters·Maria das Graças de LunaIan R Henderson
Sep 13, 2008·Annual Review of Microbiology·Marjan W van der Woude, Ian R Henderson
Aug 1, 2016·FEMS Microbiology Reviews·Satish Adhikari, Patrick D Curtis
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josep Casadesús, David Low
Jan 16, 2007·Nature Genetics·Han N Lim, Alexander van Oudenaarden
Jul 1, 2017·Proteomics·Julieanne L VoBegoña Heras
Nov 30, 2018·Molecular Microbiology·Muhammad YasirDouglas F Browning
Jul 20, 2004·Clinical Microbiology Reviews·Marjan W van der Woude, Andreas J Bäumler
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Ian R HendersonDlawer Ala'Aldeen
Apr 23, 2009·Microbiology·Rebecca Munk Vejborg, Per Klemm
May 28, 2015·Journal of Bacteriology·Kyle A TiptonPhilip N Rather
Feb 1, 2006·FEMS Microbiology Letters·Marjan W van der Woude
Aug 10, 1999·Journal of Bacteriology·H HasmanP Klemm
Dec 1, 1996·FEMS Immunology and Medical Microbiology·P OwenI Henderson
Oct 21, 1998·Trends in Microbiology·I R HendersonJ P Nataro
Nov 1, 2005·EcoSal Plus·Christophe BeloinJean-Marc Ghigo
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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