A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzyme

Molecular and Cellular Biology
Catherine M O'Connor, Kathleen Collins

Abstract

Telomerase reverse transcriptase (TERT) and telomerase RNA (TER) assemble as part of a holoenzyme that synthesizes telomeric repeats at chromosome ends. Genetic approaches have identified proteins that are required for in vivo association of TERT and TER, including the Tetrahymena telomerase holoenzyme protein p65. Here, we use quantitative assays to define the mechanisms underlying p65 function in holoenzyme biogenesis. We demonstrate that four modules of p65 contribute affinity for TER, including a C-terminal domain that recognizes the conserved dinucleotide bulge of central stem IV. This C-terminal domain is necessary and sufficient for p65's function in enhancing the recruitment of TERT to TER. Finally, we show that p65 and TERT assemble on TER with hierarchical rather than cooperative binding. These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis.

References

Apr 11, 1995·Nucleic Acids Research·M McCormick-Graham, D P Romero
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·K Collins, L Gandhi
Dec 22, 1998·Molecular and Cellular Biology·J R MitchellK Collins
May 9, 2001·The Journal of Biological Chemistry·U M OhndorfP Sondermann
Feb 19, 2002·Oncogene·Kathleen Collins, James R Mitchell
Jun 5, 2002·Annual Review of Biochemistry·Sandra L Wolin, Tommy Cedervall
Nov 6, 2002·Trends in Biochemical Sciences·Colleen KelleherJoachim Lingner
May 14, 2003·Biochemistry·Stefan AignerThomas R Cech
Jun 25, 2003·Molecular Cell·Cary K LaiKathleen Collins
Apr 15, 2004·Trends in Biochemical Sciences·Jiunn-Liang Chen, Carol W Greider
May 8, 2004·Genes & Development·Keren L Witkin, Kathleen Collins
Feb 8, 2005·Nature Structural & Molecular Biology·Ramadevi PrathapamKathleen Collins
Feb 26, 2005·The Journal of Biological Chemistry·Catherine M O'ConnorKathleen Collins

❮ Previous
Next ❯

Citations

Apr 5, 2013·Nature·Jiansen JiangJuli Feigon
Jul 11, 2006·Nature Reviews. Molecular Cell Biology·Kathleen Collins
Apr 6, 2007·Nature Structural & Molecular Biology·M Teresa Teixeira, Eric Gilson
May 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mariana MihalusovaXiaowei Zhuang
Jan 16, 2007·Molecular and Cellular Biology·Keren L WitkinKathleen Collins
Mar 23, 2011·Biochemistry. Biokhimii︠a︡·M I ZverevaO A Dontsova
Nov 20, 2012·Biochemistry. Biokhimii︠a︡·E M SmekalovaO A Dontsova
Aug 10, 2012·RNA·Emily D Egan, Kathleen Collins
Aug 6, 2011·Current Opinion in Chemical Biology·Kathleen Collins
Feb 9, 2010·Biochimica Et Biophysica Acta·Mark A BayfieldRichard J Maraia
Oct 11, 2008·Current Opinion in Chemical Biology·Elvin A AlemánDavid Rueda
Dec 7, 2007·Mechanisms of Ageing and Development·Kathleen Collins
Jun 17, 2006·The EMBO Journal·Yu ChenGabriele Varani
May 23, 2006·Current Opinion in Structural Biology·Carla A Theimer, Juli Feigon
Feb 11, 2014·Current Opinion in Structural Biology·Edward J MiraccoJuli Feigon
Jan 19, 2013·RNA Biology·Mahavir SinghJuli Feigon
Nov 14, 2006·Structure·Jason D Legassie, Michael B Jarstfer
Sep 30, 2016·Nucleic Acids Research·Catherine D EichhornJuli Feigon
Dec 3, 2016·Nucleic Acids Research·Darian D Cash, Juli Feigon
Mar 17, 2017·Annual Review of Biophysics·Henry ChanJuli Feigon
Feb 10, 2018·Nature Communications·Diego J Páez-MoscosoPeter Baumann
Jan 25, 2018·International Journal of Molecular Sciences·Abhishek Dey, Kausik Chakrabarti
Feb 10, 2018·Nature Communications·Laura C CollopyKazunori Tomita
Apr 25, 2019·Cell Cycle·Ilaria LaudadioValerio Fulci
May 14, 2020·RNA Biology·Daniele HaslerUtz Fischer
Aug 28, 2019·Cold Spring Harbor Perspectives in Biology·Yaqiang WangJuli Feigon
Dec 31, 2020·Cell Reports·Xichan HuFeng Qiao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.