A novel role for CD46 in wound repair.

Frontiers in Immunology
J CardoneClaudia Kemper

Abstract

The intestinal epithelium not only provides a vital physical barrier between the host and environment but is also required for uptake of nutrients and the induction of tolerance against commensals. Deregulation of any of these functions leads to several disease states including chronic infection, inflammatory bowel disease, and cancer. Here, we describe a novel role for the complement regulator CD46 in the regulation of intestinal epithelial cell (IEC) barrier function. We found that CD46 directly interacts in IECs with the cytoplasmic kinase SPAK and with transmembrane E-cadherin, both proteins necessary for epithelial cell junction and barrier formation. Further, CD46 activation on Caco-2 cells induced rapid and significant decrease in transepithelial resistance with concomitant increase in paracellular permeability. Importantly, though CD46 activation of IEC layers allowed for increased transgression of pathogenic E. coli, it also increased epithelial cell proliferation and accelerated wound repair. These data suggest a previously unappreciated role for CD46 in the maintenance of epithelial cell barrier integrity as well as barrier repair. However, this role for CD46 as "gate keeper" of the epithelium could also provide reas...Continue Reading

Citations

Oct 15, 2013·The International Journal of Biochemistry & Cell Biology·Hidekazu YamamotoClaudia Kemper
Oct 23, 2012·Nature Immunology·Gaëlle Le FriecClaudia Kemper
Oct 27, 2016·Immunological Reviews·Simon FreeleyGaëlle Le Friec
May 17, 2014·Inflammatory Bowel Diseases·Umang JainAndrew W Stadnyk
Jun 18, 2019·Journal of Tissue Engineering and Regenerative Medicine·Pascale DesjardinsSylvain L Guérin
Apr 30, 2019·Xenotransplantation·Hongmin ZhouDavid K C Cooper
May 27, 2016·The Journal of Biological Chemistry·Sze Jing TangXavier Roca
Apr 17, 2014·Biomedicines·Markus Vähä-Koskela, Ari Hinkkanen
Jun 4, 2020·Cellular Oncology (Dordrecht)·Łukasz ZadkaPiotr Dzięgiel

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Methods Mentioned

BETA
IECs
transgenic
IEC
Assay
Fluorescence
immunoprecipitation

Software Mentioned

MetMorph
Excel
MetaMorph
VenturiOne

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