DOI: 10.1101/508572Dec 31, 2018Paper

A novel role for Cyclic Nucleotide-Gated Ion Channel 2 (DND1) in auxin signaling

BioRxiv : the Preprint Server for Biology
Sonhita ChakrabortyKeiko Yoshioka

Abstract

Cyclic Nucleotide Gated Ion Channels (CNGCs) are non-selective cation channels that are involved in regulating responses to both biotic and abiotic stresses in plants. CNGC2 has been implicated in plant immunity and Ca2+ signaling through the study of the autoimmune phenotypes exhibited by the null mutant, defense, no death1 (dnd1) . However, dnd1 also shows additional phenotypes that are unique among autoimmune mutants. This suggests that CNGC2 plays multiple biological roles beyond pathogen defense. In this study, we cloned the gene that encodes the first suppressor of dnd1 (cngc2), REPRESSOR OF DEFENSE, NO DEATH1 (RDD1) , which encodes an auxin biosynthesis gene, YUCCA6 (YUC6) . We found that dnd1 (cngc2) is defective in auxin-mediated root growth inhibition and gravitropic responses in roots. Consistently with these auxin resistance phenotypes, we found dnd1 shows a dampened response to exogenous auxin compared to wildtype plants using the auxin inducible DR5::GUS and DII:VENUS reporter systems. Finally, auxin-induced Ca2+ influx was examined using the Forster resonance energy transfer (FRET)-based, genetically encoded Ca2+ indicator yellow cameleon (YC)-Nano65. We captured severe defects in auxin-induced Ca2+ increase in d...Continue Reading

Related Concepts

Autoimmune Diseases
Auxins
Genes
Plant Roots
Gravitropism
Gene Function
Cyclic Nucleotide-Gated Cation Channels
Calcium ion
Fluorescence Resonance Energy Transfer
CNGC2 protein, Arabidopsis

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