A novel role for Rab5-GDI in ligand sequestration into clathrin-coated pits

Current Biology : CB
H McLauchlanE Smythe

Abstract

Clathrin-coated pits are formed at the plasma membrane by the assembly of the coat components, namely clathrin and adaptors from the cytosol. Little is known about the regulation and mechanism of this assembly process. We have used an in vitro assay for clathrin-coated pit assembly to identify a novel component required for the invagination of newly formed coated pits. We have purified this cytosolic component and shown it to be a complex of Rab5 and GDI (guanine-nucleotide dissociation inhibitor), that was previously demonstrated to be involved in downstream processing of endocytic vesicles. Using a combination of quantitative electron microscopy and in vitro endocytosis assays, we have demonstrated that although coat proteins and ATP are sufficient to increase the number of new coated pits at the cell surface in permeabilised cells, the Rab5-GDI complex is required for ligand sequestration into clathrin-coated pits. We have identified Rab5 as a critical cytosolic component required for clathrin-coated pit function. Given the well-established role of Rab5 in the fusion of endocytic vesicles with endosomes, our results suggest that recruitment of essential components of the targeting and fusion machinery is coupled to the forma...Continue Reading

References

Sep 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S L Schmid
Dec 1, 1992·The Journal of Cell Biology·E SmytheS L Schmid
Dec 18, 1991·European Journal of Biochemistry·E Smythe, G Warren
Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Sep 1, 1991·The Journal of Cell Biology·S L Schmid, E Smythe
Jan 1, 1990·Annual Review of Biochemistry·J H Keen
Jan 1, 1990·Annual Review of Cell Biology·B M Pearse, M S Robinson
Jan 1, 1989·Annual Review of Cell Biology·J Gruenberg, K E Howell
Mar 1, 1989·The Journal of Cell Biology·E SmytheG Warren
Jan 1, 1986·The Journal of Cell Biology·M S Robinson, B M Pearse
Dec 1, 1986·The Journal of Cell Biology·J M LarkinR G Anderson
Mar 1, 1980·The Journal of Cell Biology·J Heuser
Jul 21, 1995·The Journal of Biological Chemistry·S R PfefferT Soldati
Sep 1, 1995·The Journal of Cell Biology·S JonesN Segev
Feb 1, 1994·The Journal of Cell Biology·E SmytheS E Moss
Jan 1, 1994·Annual Review of Biochemistry·C Nuoffer, W E Balch
Dec 15, 1994·Nature·J P LianS Ferro-Novick
Dec 1, 1993·The Journal of Cell Biology·M N SeamanM S Robinson
Aug 1, 1993·Current Opinion in Cell Biology·M Zerial, H Stenmark
Jan 1, 1993·The Journal of Cell Biology·L L CarterS L Schmid
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
Jul 11, 1996·Nature·C LamazeS L Schmid
May 23, 1997·Science·J E Rothman, T H Söllner
Aug 1, 1997·Current Biology : CB·P WiggeH T McMahon
Aug 1, 1997·Current Opinion in Cell Biology·P Novick, M Zerial

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Citations

Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·J HuangJ M Olefsky
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·R S DevonM R Hayden
Aug 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bianka L GrosshansPeter Novick
Apr 3, 2012·Annual Review of Biochemistry·Emi Mizuno-YamasakiPeter Novick
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rubén C AguilarBeverly Wendland
Jul 17, 2001·Current Opinion in Cell Biology·N Segev
May 17, 2005·Nature Cell Biology·Miyuki SatoBarth D Grant
Jun 2, 2005·Nature Cell Biology·Alexander M van der Bliek
Jun 29, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew BrighouseMark C Field
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Shannon L Compton, Ellen N Behrend
Nov 1, 2001·Annual Review of Cell and Developmental Biology·F M BrodskyD E Wakeham
May 3, 2002·Molecular and Biochemical Parasitology·Tim R JeffriesMark C Field
Oct 16, 2009·Histochemistry and Cell Biology·Catherine Sem WegnerAndreas Brech
Apr 4, 2002·Traffic·Eugeni V Entchev, Marcos A González-Gaitán
May 20, 1999·Molecular Membrane Biology·K Mohrmann, P van der Sluijs
Jan 14, 2004·The Journal of Cell Biology·Rebecca M HenryJoel A Swanson
Jun 24, 2015·Pharmaceutics·Antonietta VilellaGiovanni Tosi
Nov 5, 2008·The Journal of Cell Biology·Sophia SemerdjievaElizabeth Smythe
May 14, 2003·The Journal of Cell Biology·Tanja WucherpfennigMarcos González-Gaitán
Oct 29, 2003·The Journal of Cell Biology·Antony P JacksonElizabeth Smythe
Mar 6, 2002·The Journal of Cell Biology·Sean D Conner, Sandra L Schmid
Jun 19, 2002·The Journal of Cell Biology·Pascal SteinerHarald Hirling
Mar 27, 2001·The Journal of Cell Biology·E HillE Smythe
Jan 1, 2003·The American Journal of Pathology·Barry W McCollKaren Horsburgh
Oct 13, 2009·Biochemical and Biophysical Research Communications·Jing-Fang Wang, Kuo-Chen Chou
Aug 18, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jukka VääräniemiH Kalervo Väänänen
Sep 7, 2006·Cellular Microbiology·Stella E AutenriethIngo B Autenrieth
Sep 29, 2011·Cytoskeleton·Mathieu ChansardMinh Dang Nguyen
Nov 8, 2001·Experimental Cell Research·L LanzettiG Scita
Jan 23, 2015·Development, Growth & Differentiation·Robert FarkašBernard M Mechler

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