A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis

PloS One
Michal Shoshkes CarmelJoel K Yisraeli

Abstract

VICKZ (IGF2BP1,2,3/ZBP1/Vg1RBP/IMP1,2,3) proteins bind RNA and help regulate many RNA-mediated processes. In the midbrain region of early chick embryos, VICKZ is expressed in the neural folds and along the basal surface of the neural epithelium, but, upon neural tube closure, is down-regulated in prospective cranial neural crest (CNC) cells, concomitant with their emigration and epithelial-to-mesenchymal transition (EMT). Electroporation of constructs that modulate cVICKZ expression demonstrates that this down-regulation is both necessary and sufficient for CNC EMT. These results suggest that VICKZ down-regulation in CNC cell-autonomously promotes EMT and migration. Reduction of VICKZ throughout the embryo, however, inhibits CNC migration non-cell-autonomously, as judged by transplantation experiments in Xenopus embryos. Given the positive role reported for VICKZ proteins in promoting cell migration of chick embryo fibroblasts and many types of cancer cells, we have begun to look for specific mRNAs that could mediate context-specific differences. We report here that the laminin receptor, integrin alpha 6, is down-regulated in the dorsal neural tube when CNC cells emigrate, this process is mediated by cVICKZ, and integrin alpha ...Continue Reading

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Citations

Jun 30, 2018·Journal of Hematology & Oncology·Xinwei HuangXiangyang Kong
Feb 1, 2019·Oncogene·Yifat Bar-Zakay RosenfeldJoel K Yisraeli
Apr 14, 2020·Current Pharmaceutical Design·Peng-Fei WangShu-Lin Liu
Jul 13, 2021·Investigational New Drugs·Qiu-Ying DuDong-Sheng Pei

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Datasets Mentioned

BETA
AJ719686

Methods Mentioned

BETA
Assay
in vitro transcription
Immunoprecipitation
PCR
delamination
electrophoresis
immunoprecipitations
co-immunoprecipitation

Software Mentioned

Image J

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