A novel 'sandwich' assay for quantifying chemo-regulated cell migration within 3-dimensional matrices: wound healing cytokines exhibit distinct motogenic activities compared to the transmembrane assay

Cell Motility and the Cytoskeleton
S L SchorA M Schor

Abstract

The extracellular matrix profoundly affects cellular response to soluble motogens. In view of this critical aspect of matrix functionality, we have developed a novel assay to quantify chemo-regulated cell migration within biologically relevant 3-dimensional matrices. In this "sandwich" assay, target cells are plated at the interface between an upper and lower matrix compartment, either in the presence of an isotropic (uniform) or anisotropic (gradient) spatial distribution of test motogen. Cell migration in response to the different conditions is ascertained by quantifying their subsequent disposition within the upper and lower matrix compartments. The objective of this study has been to compare the motogenic activities of platelet-derived growth factor (PDGF-AB) and transforming growth factor-beta isoforms (TGF-beta1, -beta2 and -beta3) in the sandwich assay and the commonly employed transmembrane assay. As previously reported, dermal fibroblasts exhibited a motogenic response to isotropic and anisotropic distributions of all tested cytokines in the transmembrane assay. In contrast, only PDGF-AB and TGF-beta3 were active in the sandwich assay, each eliciting directionally unbiased (symmetrical) migration into the upper and low...Continue Reading

References

Jun 1, 1991·The Journal of Cell Biology·C Nathan, M Sporn
Jun 15, 1990·Biochemical and Biophysical Research Communications·N KoyamaS Yoshida
Jan 1, 1986·Annual Review of Medicine·S H Zigmond, D A Lauffenburger
Jan 1, 1988·Cell Motility and the Cytoskeleton·R T TranquilloD A Lauffenburger
Jan 1, 1987·The Journal of Experimental Medicine·A E PostlethwaiteA H Kang
Feb 1, 1982·The Journal of Cell Biology·H SeppäG R Martin
Jan 1, 1994·Progress in Growth Factor Research·S L Schor
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·A R Osornio-VargasA R Brody
Jan 1, 1996·The Journal of Cell Biology·J Xu, R A Clark
Apr 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R Bischoff
Jan 24, 1998·Journal of Neuro-oncology·M R Chicoine, D L Silbergeld
Sep 18, 1998·Bulletin of Mathematical Biology·A R Anderson, M A Chaplain
Oct 6, 1998·Journal of Immunological Methods·P C Wilkinson
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·P Friedl, E B Bröcker
Nov 10, 2000·Molecular Biology of the Cell·M BaillyJ E Segall
Dec 21, 2000·The Journal of Cell Biology·J M HaughT Meyer
Mar 29, 2001·International Journal of Cancer. Journal International Du Cancer·L Rönnstrand, C H Heldin
Aug 16, 2001·Critical Reviews in Toxicology·E J Calabrese
Nov 28, 2002·Biomedizinische Technik. Biomedical Engineering·R P KeatchC Matthew
Jul 5, 2003·Physiological Reviews·Sabine Werner, Richard Grose
Sep 4, 2003·The Journal of Cell Biology·Stephan J KempiakJeffrey E Segall
Dec 23, 2003·Differentiation; Research in Biological Diversity·Teresa PellegrinoCarolyn A Larabell
Mar 24, 2004·Current Opinion in Cell Biology·Carole A Parent
Sep 24, 2004·Experimental Cell Research·Shur-Jen WangNoo Li Jeon
Oct 2, 2004·The European Respiratory Journal·K ParameswaranP G Cox
Jan 7, 2005·Journal of Biomolecular Screening·Vladimir MastyuginFrank Buxton
Jan 27, 2005·Assay and Drug Development Technologies·Gillian R RichardsPeter B Simpson
Nov 18, 2005·Methods : a Companion to Methods in Enzymology·Aline ValsterMarc Symons

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Citations

Apr 28, 2009·Journal of Mammary Gland Biology and Neoplasia·Kathleen C Flanders, Lalage M Wakefield
Nov 27, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Leslie I GoldJoanne E Murphy-Ullrich
Jul 29, 2011·Science Translational Medicine·Alexander T HillelJennifer H Elisseeff
Apr 21, 2010·Experimental Cell Research·Ian R EllisSeth L Schor
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Syed Basith AmjadMichael Edward
Jan 27, 2009·Cell Motility and the Cytoskeleton·Ektoras HadjipanayiRobert A Brown
Apr 13, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Lateef E AljoraniAna M Schor
Aug 30, 2008·The American Journal of Pathology·Lillian B NanneyLeslie I Gold
Jan 17, 2015·Journal of Periodontology·Cristina C VillarDavid L Cochran
Mar 17, 2012·Current Opinion in Biotechnology·Robert P KeatchSeth L Schor
Mar 31, 2015·Nanomedicine·José Ballester-BeltránManuel Salmerón-Sánchez
Feb 20, 2014·European Journal of Clinical Nutrition·Maryam S FarvidMonir S Farvid
Jan 13, 2009·The Biochemical Journal·Marek MichalakMichal Opas
Apr 1, 2017·Advanced Biosystems·Rui-Xin WuFa-Ming Chen
Oct 22, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jiyeon LeeTanmay P Lele

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