A novel, secreted form of human ADAM 12 (meltrin alpha) provokes myogenesis in vivo.

The Journal of Biological Chemistry
B J GilpinU M Wewer

Abstract

The ADAM (A Disintegrin And Metalloprotease) family of cell-surface proteins may have an important role in cellular interactions and in modulating cellular responses. In this report we describe a novel, secreted form of human ADAM 12 (meltrin alpha), designated ADAM 12-S (S for short), and a larger, membrane-bound form designated ADAM 12-L (L for long form). These two forms arise by alternative splicing of a single gene located on chromosome 10q26. Northern blotting demonstrated that mRNAs of both forms are abundant in human term placenta and are also present in some tumor cell lines. The ADAM 12-L transcript can also be detected in normal human adult skeletal, cardiac, and smooth muscle. Human A204 embryonal rhabdomyosarcoma cells that do not differentiate into muscle cells and do not express any form of ADAM 12 were stably transfected with an ADAM 12-S minigene encoding the disintegrin domain, the cysteine-rich domain, and the unique 34 amino acid carboxyl terminus. Nude mouse tumors derived from these transfected cells contained ectopic muscle cells of apparent mouse origin as shown by species-specific markers. These results may have potential applications in the development of muscle-directed gene and cell therapies.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ W Gray
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Apr 1, 1995·Current Biology : CB·B J Mayer, M J Eck
Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
Jan 1, 1995·Methods in Enzymology·N D Rawlings, A J Barrett
May 1, 1995·The Biochemical Journal·A C PerryL Hall
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G MylesP Primakoff
Feb 5, 1993·Journal of Molecular Biology·C Landolt-MarticorenaR A Reithmeier
Feb 1, 1996·The Journal of Cell Biology·G WeskampC P Blobel
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·D FambroughC S Goodman
Oct 1, 1996·Current Opinion in Cell Biology·A P HuovilaJ M White
Dec 15, 1996·Developmental Biology·T G Wolfsberg, J M White
Mar 1, 1997·Molecular Reproduction and Development·C M VidaeusJ C Herr
Jul 1, 1997·Nucleic Acids Research·G Edwalds-GilbertC Milcarek
Jun 1, 1997·Trends in Cell Biology·D BiglerJ M White

❮ Previous
Next ❯

Citations

Oct 26, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sabine GackMarina Schorpp-Kistner
Mar 21, 2008·Histochemistry and Cell Biology·Lars-Inge LarssonJan Fredrik Talts
Oct 27, 2006·Journal of Neural Transmission·C NadriG Agam
Feb 18, 2004·The Journal of Laboratory and Clinical Medicine·Keith M Skubitz, Amy P N Skubitz
Jun 15, 2005·Pulmonary Pharmacology & Therapeutics·Stephen T HolgateJohn W Holloway
Apr 5, 2003·Biochimica Et Biophysica Acta·Yoshiki KudoI L Sargent
Feb 1, 2000·Trends in Genetics : TIG·P Primakoff, D G Myles
Apr 13, 2000·Biology of the Cell·T DarribèreD Alfandari
Sep 23, 2000·Molecular and Cellular Endocrinology·D R McCullochA C Herington
Nov 18, 1998·Current Opinion in Cell Biology·R A Black, J M White
Apr 13, 2001·Drug Discovery Today·M L. MossR C. Andrews
Jul 28, 1999·Bone·D J DallasT M Skerry
Nov 14, 2008·Nature Reviews. Cancer·Gillian Murphy
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Brenda M OgleJeffrey L Platt
Jul 6, 2001·The Biochemical Journal·Y CaoA Zolkiewska
Oct 10, 2012·Reproduction, Fertility, and Development·María E CortinaSilvia Miranda
Feb 24, 2011·Molecular & Cellular Proteomics : MCP·C Y X'avia ChanK W Michael Siu
Jul 9, 2008·Animal Biotechnology·Y TaniguchiY Sugimoto
Dec 3, 2004·Molecular Biology of the Cell·Peggy LafusteFrançois-Jérôme Authier
Dec 17, 2010·Development, Growth & Differentiation·Juntang LinChristoph Redies
Jul 23, 1999·Annals of the New York Academy of Sciences·L KillarJ Peschon
Dec 17, 2002·Molecular and Cellular Biology·Tomohiro KurisakiAtsuko Sehara-Fujisawa
Nov 29, 2002·Molecular Pathology : MP·B L TianC J Luo
Nov 28, 2007·Annual Review of Pathology·Marc B HershensonJulian Solway
May 27, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Mohammed ShakerNariaki Matsuura
Feb 15, 2005·American Journal of Respiratory and Critical Care Medicine·Hans Michael HaitchiDonna E Davies
Jun 5, 2003·American Journal of Respiratory Cell and Molecular Biology·Shelby P UmlandRobert Ralston
Sep 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Shelby P UmlandRichard Del Mastro
Jan 27, 2004·American Journal of Respiratory Cell and Molecular Biology·Robert M PowellDonna E Davies
Feb 14, 2009·American Journal of Respiratory Cell and Molecular Biology·Cecilia EstrellaPhilippe Gosset
Jun 23, 2004·American Journal of Respiratory Cell and Molecular Biology·Benjamin A Raby, Scott T Weiss
Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·DeannaLee M Beauvais, Alan C Rapraeger
Jul 16, 2009·Reproductive Biology and Endocrinology : RB&E·Simon GrillOlav Lapaire
Nov 3, 2010·Reproductive Biology and Endocrinology : RB&E·Niels TørringBruno Darbouret

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.