A novel selectable marker based on Aspergillus niger arginase expression

Enzyme and Microbial Technology
Kashyap DaveNarayan S Punekar

Abstract

Selectable markers are valuable tools in transforming asexual fungi like Aspergillus niger. An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a novel nutritional marker/selection for transformation. The development of such a marker was successfully achieved in two steps. The single genomic copy of A. niger arginase gene was disrupted by homologous integration of the bar marker. The agaA disruptant was subsequently complemented by transforming it with agaA expression vectors. Both citA and trpC promoters were able to drive the expression of arginase cDNA. Such agaA+ transformants displayed arginase expression pattern distinct from that of the parent strain. The results are also consistent with a single catabolic route for arginine in this fungus. A simple yet novel arginine-based selection for filamentous fungal transformation is thus described.

References

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Feb 10, 2006·Cell Research·Richard J WeldHayley J Ridgway
Feb 6, 2007·Journal of Biotechnology·Vera MeyerArthur F J Ram
May 16, 2008·Fungal Genetics and Biology : FG & B·Manmeet Ahuja, Narayan S Punekar
Oct 9, 2008·Biotechnology Advances·David Lubertozzi, Jay D Keasling
Jul 5, 2011·Journal of Biotechnology·Kashyap Dave, Narayan S Punekar

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Citations

Dec 19, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sarita Keni, Narayan S Punekar
Jun 7, 2015·Applied and Environmental Microbiology·Sunil KumarNarayan S Punekar
Feb 27, 2019·Microbiology·Tejaswani SaragadamNarayan S Punekar
Jun 23, 2019·Applied Microbiology and Biotechnology·Yan-Zhen MeiChuan-Chao Dai
Mar 10, 2017·BMC Microbiology·Markus R M FiedlerVera Meyer

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