A novel selective progesterone receptor modulator asoprisnil activates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated signaling pathway in cultured human uterine leiomyoma cells in the absence of comparable effects on myometrial cells

The Journal of Clinical Endocrinology and Metabolism
Hiroko SasakiT Maruo


We previously demonstrated that asoprisnil, a selective progesterone receptor modulator, induces apoptosis of cultured uterine leiomyoma cells. This study was conducted to evaluate whether asoprisnil activates TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptotic pathway in cultured uterine leiomyoma and matching myometrial cells. After subculture in phenol red-free DMEM supplemented with 10% fetal bovine serum for 120 h, cultured cells were stepped down to serum-free conditions for 24 h in the absence or presence of graded concentrations of asoprisnil. The levels of TRAIL signaling molecules and cellular inhibitors of apoptosis protein were assessed by Western blot analysis. TRAIL contents in untreated cultured leiomyoma cells were significantly (P < 0.01) lower compared with those in untreated cultured myometrial cells. There was no difference in death receptor (DR)4 and DR5 contents between the two types of cells. Asoprisnil treatment significantly (P < 0.05) increased TRAIL, DR4, and DR5 contents in cultured leiomyoma cells in a dose-dependent manner with a cleavage of caspase-8, -7, and -3, and decreased X-linked chromosome-linked inhibitor of apoptosis protein contents. In cultured myometrial cells, however, as...Continue Reading


Nov 5, 1997·Nature Genetics·G S WuW S el-Deiry
Feb 17, 1999·Genes & Development·Q L Deveraux, J C Reed
May 14, 1999·Trends in Pharmacological Sciences·A A PieperS H Snyder
Jul 20, 1999·The Journal of Clinical Investigation·A AshkenaziR H Schwall
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erika CretneyMark J Smyth
Mar 22, 2002·Nature Reviews. Immunology·M Thome, J Tschopp
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zheng LiuBert W O'Malley
Aug 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Zhijian GaoTakeshi Maruo
Dec 18, 2002·Experimental Biology and Medicine·Susan A Leonhardt, Dean P Edwards
Nov 25, 2003·Oncogene·Shulin Wang, Wafik S El-Deiry
Feb 11, 2004·Endocrine Reviews·Carolyn L Smith, Bert W O'Malley
Feb 18, 2004·Current Cancer Drug Targets·M Saeed Sheikh, Ying Huang
May 14, 2004·Human Reproduction Update·T MaruoH Matsuo
May 26, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Sharmila Shankar, Rakesh K Srivastava
Aug 4, 2004·Cancer Research·Xiangguo LiuShi-Yong Sun
Oct 27, 2004·Cancer Science·Hideo YagitaKo Okumura

❮ Previous
Next ❯


Feb 3, 2012·The New England Journal of Medicine·Jacques DonnezUNKNOWN PEARL II Study Group
Sep 11, 2014·Human Reproduction Update·Molly B MoravekSerdar E Bulun
May 3, 2014·Expert Opinion on Pharmacotherapy·Giuseppe BenagianoIvo Brosens
Jul 11, 2012·The Journal of Pharmacy and Pharmacology·Shilpa N KaoreRaj Sharma
Nov 20, 2014·Human Reproduction Update·Bansari PatelSam Mesiano
Jan 1, 2009·Progress in Molecular Biology and Translational Science·J Julie KimSerdar E Bulun
Feb 23, 2010·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Takeshi Maruo
Jul 9, 2010·Current Opinion in Obstetrics & Gynecology·Tariqu Salman, Colin Davis
Jul 27, 2007·American Journal of Physiology. Endocrinology and Metabolism·Qin XuTakeshi Maruo
Sep 6, 2007·The Journal of Clinical Endocrinology and Metabolism·Ping YinSerdar E Bulun
Jun 25, 2015·Current Opinion in Obstetrics & Gynecology·Molly B Moravek, Serdar E Bulun
Jun 8, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fraser M RogersonPeter J Fuller
Aug 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shilpi Goenka, Sanford R Simon
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Md Soriful IslamPasquapina Ciarmela
Aug 31, 2014·Fertility and Sterility·Nathalie Chabbert-BuffetPhilippe Bouchard

❮ Previous
Next ❯

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis