A novel self-deleting retroviral vector carrying an additional sequence recognized by the viral integrase (IN)

Virus Research
C Torne-CelerC Ronfort

Abstract

During retroviral integration, the viral integrase recognizes the attachment (att) sequence (formed by juxtaposition of two LTRs ends) as the substrate of integration. We have developed a self-deleting Avian Leukosis and Sarcoma Viruses (ALSVs)-based retroviral vector carrying an additional copy of the att sequence, between neo and puro genes. We observed that: (i) the resulting NP3Catt vector was produced at neo and puro titers respectively smaller and higher than that of the parental vector devoid of the att sequence; (ii) 61% of NP3Catt proviruses were flanked by LTRs; most of them were deleted of internal sequences, probably during the reverse transcription step; (iii) 31% of clones were deleted of the whole 5' part of their genome and were flanked, in 5', by the additional att sequence and, in 3', by an LTR. Integration of these last proviruses was often imprecise with respect to the viral ends. At total, 77% of proviruses had lost the packaging signal and were not mobilizable by a replication-competent virus and 92% had lost the selectable gene in a single round of replication. Although still to improve, the att vector could be considered as an interesting new safe retroviral vector for gene transfer experiments.

References

Feb 14, 1992·Science·A Swain, J M Coffin
Mar 1, 1991·Molecular and Cellular Biology·Y M Lee, J M Coffin
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T Fujiwara, R Craigie
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P O BrownJ M Bishop
Nov 25, 1987·Nucleic Acids Research·P ThoravalV Nigon
Mar 1, 1986·Molecular and Cellular Biology·M Emerman, H M Temin
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S F YuE Gilboa
Jan 22, 1982·Nucleic Acids Research·S M Mount
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S Lee, R Craigie
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L NaldiniI M Verma
Mar 30, 2002·Nature Biotechnology·Alex J HarveyRobert Ivarie
Oct 4, 2003·Nature Reviews. Genetics·John Clark, Bruce Whitelaw
Feb 24, 2004·The Journal of Gene Medicine·Christophe Delenda
Feb 24, 2004·The Journal of Gene Medicine·Thomas GrunwaldKlaus Uberla
Feb 27, 2004·Nature·Marina Cavazzana-CalvoFulvio Mavilio
Jun 12, 2004·EMBO Reports·Michael J McGrewHelen Sang
Aug 18, 2004·PLoS Biology·Rick S MitchellFrederic D Bushman
Jan 28, 2005·Transgenic Research·Alexander Pfeifer
Jan 28, 2005·Development·Susan C ChapmanGary C Schoenwolf
Apr 25, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christopher BaumMelanie Galla
Sep 29, 2006·Journal of Virology·Jangsuk OhStephen H Hughes
Jan 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·S G LillicoH M Sang
Aug 9, 2007·Physiological Genomics·Frank Park
Sep 18, 2007·Molecular Biotechnology·Adam S Cockrell, Tal Kafri

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Citations

Nov 20, 2008·Archives of Virology·Caroline Torne-CelerCorinne Ronfort

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