A novel serine protease with human fibrino(geno)lytic activities from Artocarpus heterophyllus latex

Biochimica Et Biophysica Acta
Jaruwan SiritapetaweeSompong Thammasirirak

Abstract

A protease was isolated and purified from Artocarpus heterophyllus (jackfruit) latex and designated as a 48-kDa antimicrobial protease (AMP48) in a previous publication. In this work, the enzyme was characterized for more biochemical and medicinal properties. Enzyme activity of AMP48 was strongly inhibited by phenylmethanesulfonyl fluoride and soybean trypsin inhibitor, indicating that the enzyme was a plant serine protease. The N-terminal amino acid sequences (A-Q-E-G-G-K-D-D-D-G-G) of AMP48 had no sequence similarity matches with any sequence databases of BLAST search and other plant serine protease. The secondary structure of this enzyme was composed of high α-helix (51%) and low β-sheet (9%). AMP48 had fibrinogenolytic activity with maximal activity between 55 and 60°C at pH 8. The enzyme efficiently hydrolyzed α followed by partially hydrolyzed β and γ subunits of human fibrinogen. In addition, the fibrinolytic activity was observed through the degradation products by SDS-PAGE and emphasized its activity by monitoring the alteration of secondary structure of fibrin clot after enzyme digestion using ATR-FTIR spectroscopy. This study presented the potential role to use AMP48 as antithrombotic for treatment thromboembolic dis...Continue Reading

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Citations

Mar 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yamara A S de MenezesMatheus de F Fernandes-Pedrosa
Jul 6, 2014·Pharmacognosy Magazine·Omana Sukumaran Bindhu, Maheshwari Kumari Singh
Oct 16, 2014·International Journal of Biological Macromolecules·Dae-Won KimSung-Jun Kim
Sep 16, 2016·Biotechnology Research International·Rayane Natshe GonçalvesRaquel Elisa da Silva-López
Oct 30, 2016·Bioengineered· Adivitiya, Yogender Pal Khasa
Nov 28, 2017·Current Medicinal Chemistry·Anastasia V BalakirevaAndrey A Zamyatnin
Jan 20, 2016·The Journal of General and Applied Microbiology·Palaniyandi VelusamyJae-Seong So
Jun 15, 2021·Frontiers in Molecular Biosciences·Farwa AltafVivi Kasim

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