A novel signal amplification strategy based on the use of copper nanoclusters for ratiometric fluorimetric determination of o-phenylenediamine

Mikrochimica Acta
Yujun MaManli Guo

Abstract

Copper nanoclusters (CuNCs) were synthesized starting from glutathione and copper(II) nitrate. They show blue fluorescence peaking at 432 nm when excited at 334 nm. In the presence of o-phenylenediamine (OPD), the blue fluorescence is decreased, but a yellow fluorescence appears with a peak at 557 nm. UV-visible absorptiometry, fluorometry and fluorescence lifetime measurements were used to show that OPD is oxidized by the small fraction of copper(II) ions present in the CuNCs to form the oxidized form of o-phenylenediamine (oxOPD) which displays weak yellow fluorescence, while the blue fluorescence decreases. The ratio of fluorescences at 557 and 432 nm increases linearly in the 0.15 to 110 μg·L-1 OPD concentration range, and the detection limit is 93 ng·L-1. Compared to the method based on the use of dissolved Cu(II), the employment of CuNCs reduces the detection limit by a factor of 40. The method was applied to the determination of OPD in spiked environmental water and industrial wastewater samples. Recoveries ranged from 96.8 to 100.3%. Graphical abstract Schematic presentation of a ratiometric fluorometric method for detection of o-phenylenediamine (OPD) based on copper nanoclusters (CuNCs). OPD is oxidized by Cu2+ presen...Continue Reading

References

Jan 24, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nan LiHua He
Jul 10, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ru LiHua Wang
Dec 20, 2017·Analytica Chimica Acta·Shuai ChenJian-Hua Wang
Feb 13, 2018·ACS Applied Materials & Interfaces·Mingshu XiaoJiang Li

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