A novel signaling network essential for regulating Pseudomonas aeruginosa biofilm development.

PLoS Pathogens
Olga E Petrova, Karin Sauer

Abstract

The important human pathogen Pseudomonas aeruginosa has been linked to numerous biofilm-related chronic infections. Here, we demonstrate that biofilm formation following the transition to the surface attached lifestyle is regulated by three previously undescribed two-component systems: BfiSR (PA4196-4197) harboring an RpoD-like domain, an OmpR-like BfmSR (PA4101-4102), and MifSR (PA5511-5512) belonging to the family of NtrC-like transcriptional regulators. These two-component systems become sequentially phosphorylated during biofilm formation. Inactivation of bfiS, bfmR, and mifR arrested biofilm formation at the transition to the irreversible attachment, maturation-1 and -2 stages, respectively, as indicated by analyses of biofilm architecture, and protein and phosphoprotein patterns. Moreover, discontinuation of bfiS, bfmR, and mifR expression in established biofilms resulted in the collapse of biofilms to an earlier developmental stage, indicating a requirement for these regulatory systems for the development and maintenance of normal biofilm architecture. Interestingly, inactivation did not affect planktonic growth, motility, polysaccharide production, or initial attachment. Further, we demonstrate the interdependency of th...Continue Reading

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Citations

Jul 19, 2013·Future Medicinal Chemistry·Roberta J WorthingtonChristian Melander
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Dec 25, 2010·Applied Microbiology and Biotechnology·Viviana Sanchez-TorresThomas K Wood
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Methods Mentioned

BETA
immunoprecipitation
confocal microscopy
pull-down
reverse phase chromatography

Software Mentioned

BLINK
ImageMaster Platinum
cICAT
COMSTAT
ProID
ProICAT
Analyst QS
ImageMaster
Bioanalyst
2D

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