A novel silicon patch-clamp chip permits high-fidelity recording of ion channel activity from functionally defined neurons

Biotechnology and Bioengineering
Christophe PyGeoff Mealing

Abstract

We report on a simple and high-yield manufacturing process for silicon planar patch-clamp chips, which allow low capacitance and series resistance from individually identified cultured neurons. Apertures are etched in a high-quality silicon nitride film on a silicon wafer; wells are opened on the backside of the wafer by wet etching and passivated by a thick deposited silicon dioxide film to reduce the capacitance of the chip and to facilitate the formation of a high-impedance cell to aperture seal. The chip surface is suitable for culture of neurons over a small orifice in the substrate with minimal leak current. Collectively, these features enable high-fidelity electrophysiological recording of transmembrane currents resulting from ion channel activity in cultured neurons. Using cultured Lymnaea neurons we demonstrate whole-cell current recordings obtained from a voltage-clamp stimulation protocol, and in current-clamp mode we report action potentials stimulated by membrane depolarization steps. Despite the relatively large size of these neurons, good temporal and spatial control of cell membrane voltage was evident. To our knowledge this is the first report of recording of ion channel activity and action potentials from neur...Continue Reading

References

May 1, 1997·Journal of Neurophysiology·W F GillyM McFarlane
Aug 26, 1998·Methods in Enzymology·R A Levis, J L Rae
May 23, 2002·Biophysical Journal·Niels FertigJan C Behrends
Feb 3, 2004·Physical Review Letters·R Alexander KaulPeter Fromherz
Oct 21, 2004·Biosensors & Bioelectronics·Rigo PantojaJames R Heath
Dec 4, 2004·Pflügers Archiv : European journal of physiology·Kathryn G KlemicFred J Sigworth
Apr 9, 2005·IEEE Transactions on Nanobioscience·Fred J Sigworth, Kathryn G Klemic
Apr 6, 2006·Journal of Biotechnology·Thomas SordelNathalie Picollet-D'hahan
Aug 29, 2007·Analytica Chimica Acta·Yulia MourzinaAndreas Offenhäusser
Mar 22, 2008·Nature Reviews. Drug Discovery·John DunlopRobert Arias
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Harold J Bell, Naweed I Syed
Mar 1, 2007·IEEE Transactions on Biomedical Circuits and Systems·J N Y AzizP L Carlen

❮ Previous
Next ❯

Citations

Apr 28, 2012·Journal of Molecular Histology·Nichole Schmold, Naweed I Syed
Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Feb 22, 2012·Journal of Visualized Experiments : JoVE·Christophe PyGeoff Mealing
Feb 18, 2011·Biotechnology and Bioengineering·Chang-Yu ChenAndrew M Wo
May 2, 2014·New Biotechnology·Christophe PyPing Zhao
May 5, 2011·Journal of Neural Engineering·Marzia MartinaGeoffrey A R Mealing
May 23, 2014·Lab on a Chip·Brandon R BruhnMichael Mayer
Dec 17, 2010·Expert Review of Medical Devices·Geoffrey Mealing, Christophe Py
May 17, 2018·Brain and Neuroscience Advances·Luca A Annecchino, Simon R Schultz
Jun 7, 2014·Chemical Reviews·Qingjun LiuPing Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.