A Novel Silver Bioactive Glass Elicits Antimicrobial Efficacy Against Pseudomonas aeruginosa and Staphylococcus aureus in an ex Vivo Skin Wound Biofilm Model

Frontiers in Microbiology
Holly N WilkinsonMatthew J Hardman

Abstract

Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic stewardship, effective new anti-biofilm therapies are urgently needed for effective wound management. Previous studies have shown that bioactive glass (Bg) is able to promote healing with moderate bactericidal activity. Here we tested the antimicrobial efficacy of a novel BG incorporating silver (BgAg), against both planktonic and biofilm forms of the wound-relevant bacteria Pseudomonas aeruginosa and Staphylococcus aureus. BgAg was stable, long lasting, and potently effective against planktonic bacteria in time-kill assays (6-log reduction in bacterial viability within 2 h) and in agar diffusion assays. BgAg reduced bacterial load in a physiologically relevant ex vivo porcine wound biofilm model; P. aeruginosa (2-log reduction) and S. aureus (3-log reduction). BgAg also conferred strong effects against P. aeruginosa biofilm virulence, reducing both protease activity and virulence gene expression. Co-culture biofilms appeared more resistant to BgAg, where a selective reduction in S. aureus...Continue Reading

References

Aug 4, 1998·Acta Odontologica Scandinavica·P StoorJ I Salonen
Nov 7, 1998·The Journal of Biological Chemistry·E KesslerD E Ohman
Mar 10, 2001·Analytical Biochemistry·A R CaballeroR J O'Callaghan
Jun 28, 2001·Journal of Biomedical Materials Research·P Saravanapavan, L L Hench
Oct 11, 2001·Microbiology and Immunology·T ShigematsuK Okuda
Mar 24, 2004·Current Opinion in Microbiology·Andrew L Goodman, Stephen Lory
Dec 4, 2004·Journal of Bacteriology·Brian WeinrickRichard P Novick
Jan 5, 2005·Journal of Bacteriology·Lauren M MashburnMarvin Whiteley
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeff G LeidAlyssa K Jeffers
Mar 21, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Matthias ZehnderEva Söderling
Nov 24, 2006·Journal of Materials Science. Materials in Medicine·Larry L Hench
Apr 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kelly C RiceKenneth W Bayles
Jun 15, 2007·Journal of Materials Science. Materials in Medicine·Eveliina MunukkaErkki Eerola
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Feb 6, 2008·Acta Biomaterialia·Jayesh P RupareliaSuparna Mukherji
Aug 26, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·S SchremlP Babilas
Sep 1, 2009·Applied and Environmental Microbiology·Lalitha BiswasFriedrich Götz
Nov 26, 2009·The Journal of International Medical Research·Tomaz VelnarV Smrkolj
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
Feb 11, 2011·Journal of Visualized Experiments : JoVE·George A O'Toole
May 11, 2011·Journal of Materials Science. Materials in Medicine·Carlos Henrique Gomes MartinsLuciana Assirati Casemiro
Jun 15, 2011·Trends in Microbiology·Elena B M BreidensteinRobert E W Hancock
Jun 28, 2012·Biomedical Materials·Cai LinXiaofeng Chen
Jul 17, 2012·Journal of Bacteriology·Thien-Fah Mah
Aug 28, 2012·Acta Biomaterialia·Julian R Jones
Aug 30, 2012·Critical Reviews in Microbiology·Jean-Yves Maillard, Philippe Hartemann
Sep 4, 2012·Trends in Biotechnology·Susmita BoseAmit Bandyopadhyay
Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aishwarya KorgaonkarMarvin Whiteley
Apr 27, 2013·Pathogens and Disease·Shaan L Gellatly, Robert E W Hancock
Jun 21, 2013·Science Translational Medicine·Jose Ruben Morones-RamirezJames J Collins

❮ Previous
Next ❯

Citations

Oct 1, 2020·Open Biology·Holly N Wilkinson, Matthew J Hardman
Jul 8, 2020·Advances in Wound Care·Varuni R BrownhillHolly N Wilkinson
Dec 4, 2020·Emerging Topics in Life Sciences·Joanna Shepherd
Dec 23, 2020·Biofouling·Débora de Souza Collares Maia Castelo-BrancoJosé Júlio Costa Sidrim
Dec 31, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Holly N WilkinsonMatthew J Hardman
Jan 23, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Brooke A PatiLisa M Pierce
Nov 14, 2019·ACS Applied Materials & Interfaces·Paul J WeldrickVesselin N Paunov
Jun 3, 2021·International Journal of Molecular Sciences·Madelene Å AnderssonManoj Puthia
Dec 16, 2020·ACS Biomaterials Science & Engineering·Tina MehrabiZahra Mohammadi
Jan 27, 2019·The Journal of Investigative Dermatology·Holly N WilkinsonMatthew J Hardman
Oct 26, 2021·Antimicrobial Agents and Chemotherapy·Devin SindeldeckerPaul Stoodley
Jan 23, 2022·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·W M Abd-Allah, Rasha Mohammad Fathy

❮ Previous
Next ❯

Methods Mentioned

BETA
zymography
biopsies

Software Mentioned

Gram
R
ImageJ
- Twort

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.