A novel single WAP domain-containing protein isoform with antibacterial relevance in Litopenaeus vannamei

Fish & Shellfish Immunology
Zhi-Qiang DuDa-Ming Ren

Abstract

Single WAP domain (SWD)-containing protein is a small protein containing a whey acidic protein (WAP) domain at the C-terminal region. SWD-containing protein exhibits structural similarity to the family of serine proteinase inhibitors. As of this writing, some SWD domain-containing proteins have been identified in crustaceans, and their functions included antibacterial and anti-proteinase activities. We identified a SWD protein isoform gene in Litopenaeus vanname (Lv-SWDi). Very high sequence similarity was found between Lv-SWDi and Lv-SWD. Results of time-course analysis for the gene expression profile showed that Lv-SWDi could produce a rapid feedback and an obvious upregulation at 12 h after Vibrio injection. Endogenous Lv-SWDi protein was obviously upregulated, and the highest expression level was reached at 24 h after Vibrio injection. The purified rLv-SWDi could directly bind to Gram-positive and Gram-negative bacteria. Results of the proteinase inhibitory assay also showed that rLv-SWDi could inhibit secretory protease activity from Bacillus subtilis. Lv-SWDi is a part of an important immunity-relevant gene and may serve important functions in defense against bacteria.

References

Oct 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Z Liang, K Söderhäll
Jul 15, 1994·European Journal of Biochemistry·M W JohanssonK Söderhäll
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z LiangK Söderhäll
Nov 14, 1997·The Journal of Biological Chemistry·D DestoumieuxE Bachère
Jul 30, 1999·Developmental and Comparative Immunology·M R Kanost
Feb 19, 2000·Journal of Molecular Graphics & Modelling·S RanganathanK R Nicholas
Feb 13, 2001·Insect Biochemistry and Molecular Biology·M J Gorman, S M Paskewitz
Jan 27, 2004·Biochemical and Biophysical Research Communications·Florinda Jiménez-VegaFrancisco Vargas-Albores
Feb 15, 2005·Developmental and Comparative Immunology·Florinda Jiménez-Vega, Francisco Vargas-Albores
Mar 8, 2006·Developmental and Comparative Immunology·Nawarat SomprasongAnchalee Tassanakajon
Jan 29, 2008·Developmental and Comparative Immunology·Valerie J SmithChris Hauton
Jul 8, 2008·Developmental and Comparative Immunology·Piti AmparyupAnchalee Tassanakajon
Aug 5, 2008·Trends in Immunology·Colin D Bingle, Annapurna Vyakarnam
Aug 30, 2008·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Xingjiang BuJinxing Wang
Sep 16, 2008·Cellular Signalling·Vassilis J Marmaras, Maria Lampropoulou
Jun 24, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhi-Qiang DuJin-Xing Wang

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Citations

Sep 1, 2015·Molecular Immunology·Valerie J Smith, Elisabeth A Dyrynda
May 11, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Delphine Destoumieux-GarzónEvelyne Bachère
Jun 26, 2020·Molecular & Cellular Proteomics : MCP·Frederik TellkampMarcus Krüger
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Frederik TellkampMarcus Krüger

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