A novel site-specific recombination system derived from bacteriophage phiMR11

Biochemical and Biophysical Research Communications
Mohammad RashelShigenobu Matsuzaki

Abstract

We report identification of a novel site-specific DNA recombination system that functions in both in vivo and in vitro, derived from lysogenic Staphylococcus aureus phage phiMR11. In silico analysis of the phiMR11 genome indicated orf1 as a putative integrase gene. Phage and bacterial attachment sites (attP and attB, respectively) and attachment junctions were determined and their nucleotide sequences decoded. Sequences of attP and attB were mostly different to each other except for a two bp common core that was the crossover point. We found several inverted repeats adjacent to the core sequence of attP as potential protein binding sites. The precise and efficient integration properties of phiMR11 integrase were shown on attP and attB in Escherichia coli and the minimum size of attP was found to be 34bp. In in vitro assays using crude or purified integrase, only buffer and substrate DNAs were required for the recombination reaction, indicating that other bacterially encoded factors are not essential for activity.

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H M Thorpe, M C Smith
Nov 15, 2000·Genome Research·J L HartleyM A Brasch
Dec 15, 2000·Molecular Microbiology·T YamaguchiM Sugai
Apr 26, 2002·Molecular Microbiology·Margaret C M Smith, Helena M Thorpe
Feb 25, 2003·The Journal of Infectious Diseases·Shigenobu MatsuzakiShosuke Imai
Jan 16, 2004·Developmental Cell·Catherine S Branda, Susan M Dymecki
Mar 9, 2005·Molecular Microbiology·Lori A BibbGraham F Hatfull
Mar 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tony KwanJerry Pelletier
May 25, 2006·PLoS Biology·Pallavi GhoshGraham F Hatfull
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice
Dec 16, 2006·Current Gene Therapy·Michele P Calos
Dec 19, 2006·Journal of Bacteriology·Anu DanielVincent A Fischetti
Oct 12, 2007·Journal of Biochemistry and Molecular Biology·Malabika DasSubrata Sau

❮ Previous
Next ❯

Citations

Nov 19, 2010·Applied Microbiology and Biotechnology·Nobutaka HiranoMitsuru Haruki
Dec 7, 2011·Current Microbiology·Jarmila Farkašovská, Andrej Godány
Aug 29, 2013·Critical Reviews in Biochemistry and Molecular Biology·Gregory D Van Duyne, Karen Rutherford
Jun 1, 2016·IEEE Transactions on Biomedical Circuits and Systems·Jack BowyerDeclan Bates
May 27, 2014·Journal of Molecular Biology·Paul C M FoggMargaret C M Smith
Mar 20, 2010·Biochemical Society Transactions·Margaret C M SmithPaul A Rowley
Oct 11, 2017·Scientific Reports·Tai-Yin Chiu, Jie-Hong R Jiang
Sep 10, 2015·Microbiology Spectrum·Margaret C M Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Journal of Bacteriology
Stephanie M StollMichele P Calos
Proceedings of the National Academy of Sciences of the United States of America
Aram AkopianW M Stark
© 2021 Meta ULC. All rights reserved