A novel somatodendritic marker defined by a peptide derived from the ALS2 protein

Neuroreport
Virginie BrosJean-Marc Gallo

Abstract

We characterized an antibody raised against a peptide derived from the ALS2 protein, the product of a gene linked to recessive juvenile forms of amyotrophic lateral sclerosis, defining the ALS2 RLD-derived antigen (ARDA). In cultured rat cortical neurons, ARDA is localized in perikarya and neurites, but absent from nuclei. Double immunostaining of rodent cultured neurons and spinal cord sections for ARDA and MAP2, a marker of dendrites, or tau and phosphorylated neurofilaments, markers of axons, demonstrated that the ARDA antigen was localized in the somatodendritic compartment and excluded from axons. ARDA was also selectively localized to the somatodendritic compartment of motor neurons in human spinal cord. The ARDA antigen therefore represents a novel somatodendritic marker for embryonic and adult neurons.

References

Jan 1, 1986·Annals of the New York Academy of Sciences·L I BinderL I Rebhun
Oct 1, 1985·The Journal of Cell Biology·L I BinderL I Rebhun
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A MatusT Hugh-Jones
Aug 25, 2001·Annual Review of Neuroscience·V M LeeJ Q Trojanowski
Jan 2, 2003·Annals of Neurology·François Gros-LouisGuy A Rouleau
Jan 7, 2003·International Review of Neurobiology·Christopher J McDermott, Pamela J Shaw
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Koji YamanakaDon W Cleveland
Mar 23, 2004·The Journal of Biological Chemistry·Justin D ToppBruce F Horazdovsky

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Citations

Aug 9, 2005·Biochimica Et Biophysica Acta·Stéphanie MillecampsJean-Pierre Julien

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