Abstract
We characterized an antibody raised against a peptide derived from the ALS2 protein, the product of a gene linked to recessive juvenile forms of amyotrophic lateral sclerosis, defining the ALS2 RLD-derived antigen (ARDA). In cultured rat cortical neurons, ARDA is localized in perikarya and neurites, but absent from nuclei. Double immunostaining of rodent cultured neurons and spinal cord sections for ARDA and MAP2, a marker of dendrites, or tau and phosphorylated neurofilaments, markers of axons, demonstrated that the ARDA antigen was localized in the somatodendritic compartment and excluded from axons. ARDA was also selectively localized to the somatodendritic compartment of motor neurons in human spinal cord. The ARDA antigen therefore represents a novel somatodendritic marker for embryonic and adult neurons.
References
Oct 1, 1991·The Journal of Cell Biology·P L CameronP De Camilli
Jan 1, 1986·Annals of the New York Academy of Sciences·L I BinderL I Rebhun
Oct 1, 1987·Neuroscience·C G DottiL I Binder
Dec 15, 1988·Nature·C C GarnerA Matus
Oct 1, 1985·The Journal of Cell Biology·L I BinderL I Rebhun
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A MatusT Hugh-Jones
Aug 25, 2001·Annual Review of Neuroscience·V M LeeJ Q Trojanowski
Oct 5, 2001·Nature Genetics·Y YangT Siddique
Oct 5, 2001·Nature Genetics·S HadanoJ E Ikeda
Jan 2, 2003·Annals of Neurology·François Gros-LouisGuy A Rouleau
Jan 7, 2003·International Review of Neurobiology·Christopher J McDermott, Pamela J Shaw
Jul 3, 2003·Human Molecular Genetics·Asako OtomoJoh-E Ikeda
Aug 16, 2003·Clinical Genetics·R S DevonM R Hayden
Sep 25, 2003·The Journal of Biological Chemistry·Peng-Peng ZhuCraig Blackstone
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Koji YamanakaDon W Cleveland
Mar 23, 2004·The Journal of Biological Chemistry·Justin D ToppBruce F Horazdovsky