A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress

Frontiers in Plant Science
Dayong ZhangHongbo Shao

Abstract

Water is essential for plant growth and development. Water deficiency leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from soybean with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% PEG6000 (polyethylene glycol) and 100 μM ABA (abscisic acid) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in soybean. In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane-localized protein. Yeast ...Continue Reading

References

Mar 1, 1994·The Plant Journal : for Cell and Molecular Biology·S Abel, A Theologis
Aug 7, 2002·Current Opinion in Structural Biology·Yoshinori FujiyoshiAndreas Engel
Jul 22, 2005·Plant & Cell Physiology·Junko SakuraiMasayoshi Maeshima
May 30, 2006·Biochimica Et Biophysica Acta·Ralf Kaldenhoff, Matthias Fischer
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Enric ZelaznyFrançois Chaumont
Apr 24, 2008·BMC Plant Biology·Jonas A H Danielson, Urban Johanson
Mar 3, 2009·Applied and Environmental Microbiology·Abul Kalam AzadHitoshi Shibata
Feb 22, 2011·Comptes rendus biologies·Ayalew LigabaGemechis Djira
Oct 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Marcela I RodriguesIvan G Maia
Nov 22, 2013·PloS One·Stefan ReuscherKatsuhiro Shiratake
Feb 4, 2014·Journal of Nucleic Acids·Manassés Daniel da SilvaEderson Akio Kido
Aug 7, 2014·Functional & Integrative Genomics·Preetom RegonSanjib Kumar Panda
Nov 29, 2014·Critical Reviews in Biotechnology·Ashish Kumar SrivastavaLam-Son Phan Tran
Mar 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Lei DingShiwei Guo
Aug 5, 2015·Plant Physiology and Biochemistry : PPB·Matilda MiniussiAndrea Nardini

❮ Previous
Next ❯

Citations

Jun 30, 2019·International Journal of Molecular Sciences·Yuan GaoYou-Zhi Ma
Jan 13, 2019·International Journal of Molecular Sciences·Zhi-Juan FengYa-Ming Gong
Jul 14, 2018·Environmental Science and Pollution Research International·Prabal DasMaumita Bandyopadhyay
Jan 12, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·A M AbdelmoghnyV N Waghmare

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FRET
transgenic
fluorescence microscopy

Software Mentioned

Clustalx
PlantCARE
MEGA
EXCEL
SMART
CARE
BLAST X

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.