A novel spin probe with long life in vivo for ESR imaging

Biological & Pharmaceutical Bulletin
H YoshiokaS Kazama

Abstract

A dextran-bonded nitroxide radical (TEMPO-DX) was synthesized to obtain a radical with long life in vivo for ESR imaging. TEMPO-DX was injected intravenously into a rat tail vein and the decrease in ESR intensity in the collected, circulating blood was followed. The result showed that the half life of TEMPO-DX in vivo was 30 min, the longest value reported so far and more than 30 times longer than the corresponding radicals of the six-membered piperidine ring, which means that the bonding of a radical to the polymer greatly prolonged life. The stabilities of TEMPO-DX against the reduction with L-ascorbic acid and the rat liver homogenate were also examined and compared with those of the 3-carbamoyl-2,2,5,5-tetramethylpyrolidin-1-yloxy (CPROXYL) known as a radical stable in vivo. TEMPO-DX was shown not to be as stable as CPROXYL, thus in vivo stability of TEMPO-DX arises from the fact that it is slowly absorbed into the tissues where the radicals are quenched. An ESR image of the mouse head domain was obtained only by an intravenous injection of TEMPO-DX solution into the tail vein.

Citations

Aug 21, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Toru YoshitomiYukio Nagasaki
May 20, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maya Ben Yehuda GreenwaldRon Kohen
Oct 1, 1996·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S KazamaL J Berliner
Sep 13, 2014·Advanced Healthcare Materials·Masaki MoriyamaUrara Hasegawa
Aug 14, 1998·Physics in Medicine and Biology·V Quaresima, M Ferrari

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