A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile

Ticks and Tick-borne Diseases
Maria OgrzewalskaMarcelo B Labruna

Abstract

The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009-2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap su...Continue Reading

References

Aug 12, 2000·International Journal of Systematic and Evolutionary Microbiology·V Roux, D Raoult
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Mar 9, 2005·Emerging Infectious Diseases·Luis A SangioniMarcelo B Labruna
Jun 23, 2009·Annals of the New York Academy of Sciences·Pierre-Edouard Fournier, Didier Raoult
Jul 9, 2009·Annals of Tropical Medicine and Parasitology·M OgrzewalskaM B Labruna
Feb 5, 2011·Emerging Infectious Diseases·Nanci SilvaAlbert I Ko
Jun 12, 2012·Journal of Medical Entomology·Richard C PachecoMarcelo B Labruna
Oct 5, 2013·Clinical Microbiology Reviews·Philippe ParolaDidier Raoult

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Citations

Sep 1, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Fernando Sebastián FloresMarcelo Bahia Labruna
Feb 19, 2017·Applied and Environmental Microbiology·Christopher D PaddockJerome Goddard
Jun 18, 2020·Tropical Medicine and Infectious Disease·Teresa TapiaJuan Carlos Hormazabal
Feb 15, 2018·Applied and Environmental Microbiology·Fernanda A Nieri-BastosMarcelo B Labruna
Nov 5, 2021·Microbiology Resource Announcements·Sandor E KarpathyDavid T Gauthier

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