A Novel SS18-SSX Fusion-specific Antibody for the Diagnosis of Synovial Sarcoma.

The American Journal of Surgical Pathology
Esther BaranovJason L Hornick

Abstract

Synovial sarcoma (SS), an aggressive soft tissue sarcoma with a predilection for the extremities of young adults, harbors the pathognomonic t(X;18)(p11;q11) translocation, resulting in SS18-SSX rearrangements. SS includes monophasic, biphasic, and poorly differentiated variants, which show considerable histologic overlap with a range of other tumor types, making the diagnosis challenging on limited biopsies. Immunohistochemistry (IHC) is routinely used in the differential diagnosis; however, presently available markers lack specificity. Thus, cytogenetic or molecular genetic techniques are often employed to confirm the diagnosis. Here, we report the development and characterization of 2 novel antibodies: an SS18-SSX fusion-specific antibody (E9X9V, designed to the breakpoint) as well as an SSX-specific antibody (E5A2C, designed to the SSX C-terminus). We validated the selectivity and specificity of the antibodies using immunoblotting, immunoprecipitation, and chromatin immunoprecipitation followed by next-generation sequencing in SS cell lines and demonstrated that both antibodies capture SS18-SSX on chromatin at established target sites (eg, TLE1 and BCL2) genome-wide. Using IHC in whole sections from 400 tumors including 100 ...Continue Reading

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Citations

Jun 22, 2020·Pathology International·Shinichiro TaharaEiichi Morii
Sep 2, 2020·Diagnostics·Joon Hyuk Choi, Jae Y Ro
Jul 18, 2020·The American Journal of Surgical Pathology·David L KolinMarisa R Nucci
Jan 1, 2021·Histopathology·David J Papke, Jason L Hornick
Feb 15, 2021·Pathology, Research and Practice·Yusuke KuboyamaYoshinao Oda
Dec 11, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David O AzorsaJaved Khan
Mar 6, 2021·Seminars in Diagnostic Pathology·Abbas Agaimy, Justin A Bishop
Mar 19, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Esther BaranovJason L Hornick
Jun 20, 2020·Neoplasia : an International Journal for Oncology Research·Ilaria Di MauroFlorence Pedeutour
Aug 18, 2020·American Journal of Ophthalmology·Tatyana MilmanRalph C Eagle
Sep 29, 2020·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Lama AlabdulaalySook-Bin Woo
Apr 13, 2021·Clinical Journal of Gastroenterology·Yuko KinowakiMasanobu Kitagawa
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Jun 1, 2021·International Journal of Surgical Pathology·Rui PanQiu Rao
Jun 7, 2021·Virchows Archiv : an International Journal of Pathology·Timothy Kwang Yong TayKenneth Tou En Chang
May 20, 2021·Advances in Anatomic Pathology·Nicholas BaniakMichelle S Hirsch
Aug 11, 2021·BMJ Case Reports·Quan DoVikas Singh
Aug 12, 2021·EFORT Open Reviews·Olga SavvidouPanayiotis J Papagelopoulos
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Nov 14, 2020·Surgical Pathology Clinics·Sonja ChenMichael A Arnold
Feb 16, 2021·Ophthalmic Plastic and Reconstructive Surgery·Kalla A GervasioTatyana Milman
Sep 11, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Akihiko YoshidaTaisuke Mori
Nov 3, 2021·Cancer Investigation·Neda KhaliliMihan Pourabdollah Toutkaboni

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Methods Mentioned

BETA
biopsies
Immunoprecipitation
ChIP-seq
coimmunoprecipitations

Software Mentioned

samtools
bedGraphToBigWig script
Bowtie2
IGV
CytoVision Genus
MACS2

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