Abstract
Synovial sarcoma (SS), an aggressive soft tissue sarcoma with a predilection for the extremities of young adults, harbors the pathognomonic t(X;18)(p11;q11) translocation, resulting in SS18-SSX rearrangements. SS includes monophasic, biphasic, and poorly differentiated variants, which show considerable histologic overlap with a range of other tumor types, making the diagnosis challenging on limited biopsies. Immunohistochemistry (IHC) is routinely used in the differential diagnosis; however, presently available markers lack specificity. Thus, cytogenetic or molecular genetic techniques are often employed to confirm the diagnosis. Here, we report the development and characterization of 2 novel antibodies: an SS18-SSX fusion-specific antibody (E9X9V, designed to the breakpoint) as well as an SSX-specific antibody (E5A2C, designed to the SSX C-terminus). We validated the selectivity and specificity of the antibodies using immunoblotting, immunoprecipitation, and chromatin immunoprecipitation followed by next-generation sequencing in SS cell lines and demonstrated that both antibodies capture SS18-SSX on chromatin at established target sites (eg, TLE1 and BCL2) genome-wide. Using IHC in whole sections from 400 tumors including 100 ...Continue Reading
References
Jun 25, 1998·International Journal of Cancer. Journal International Du Cancer·O TüreciM Pfreundschuh
Dec 7, 2000·Genes, Chromosomes & Cancer·N R dos SantosA G van Kessel
Nov 1, 2002·The American Journal of Surgical Pathology·Manuela PelmusJean-Michel Coindre
Mar 11, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Stephen H OlsenDavid R Lucas
Jan 27, 2007·The American Journal of Surgical Pathology·Jefferson TerryTorsten O Nielsen
Mar 6, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Maria Fernanda C AmaryAdrienne M Flanagan
Sep 17, 2008·Nature·Ron FiresteinWilliam C Hahn
Apr 14, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kemal KosemehmetogluAndrew L Folpe
Jun 11, 2009·Cancer·Iyad SultanAndrea Ferrari
Oct 20, 2009·Cancer Genetics and Cytogenetics·Euthimios DimitriadisTheoni Trangas
Mar 23, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kenichi KohashiMasazumi Tsuneyoshi
Sep 22, 2010·Virchows Archiv : an International Journal of Pathology·Atsuji MatsuyamaHiroshi Hashimoto
Mar 19, 2011·The American Journal of Surgical Pathology·Leona A DoyleJason L Hornick
May 17, 2011·American Journal of Clinical Pathology·Wai Chin FooJason L Hornick
Dec 19, 2012·Human Pathology·Michael A ArnoldMaria Tsokos
Apr 2, 2013·Cell·Cigall Kadoch, Gerald R Crabtree
Sep 14, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Leona A DoyleJason L Hornick
Dec 3, 2014·Annals of Diagnostic Pathology·Khin Thway, Cyril Fisher
Sep 29, 2015·The American Journal of Surgical Pathology·Leona A DoyleJason L Hornick
Feb 6, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yin P HungJason L Hornick
Oct 19, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Daniel J ZaccariniShengle Zhang
Nov 25, 2016·JCI Insight·Lynette M ShollLaura E MacConaill
Dec 24, 2016·The American Journal of Surgical Pathology·Yin P HungJason L Hornick
Jan 24, 2018·The Journal of Pathology·Matthew J McBride, Cigall Kadoch
Feb 13, 2018·The Journal of Obstetrics and Gynaecology Research·Yuan LiLiping Han
Jun 5, 2018·Cancer Cell·Matthew J McBrideCigall Kadoch
Jan 11, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vignesh ShanmugamJason L Hornick
Citations
Jun 22, 2020·Pathology International·Shinichiro TaharaEiichi Morii
Jun 20, 2020·Histopathology·Matthew ZaborowskiAnthony J Gill
Sep 2, 2020·Diagnostics·Joon Hyuk Choi, Jae Y Ro
Jul 18, 2020·The American Journal of Surgical Pathology·David L KolinMarisa R Nucci
Jan 1, 2021·Histopathology·David J Papke, Jason L Hornick
Feb 15, 2021·Pathology, Research and Practice·Yusuke KuboyamaYoshinao Oda
Dec 11, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David O AzorsaJaved Khan
Mar 6, 2021·Seminars in Diagnostic Pathology·Abbas Agaimy, Justin A Bishop
Nov 26, 2020·The American Journal of Surgical Pathology·Raul PerretFrançois Le Loarer
Jan 6, 2021·The American Journal of Surgical Pathology·Jason L Hornick
Mar 19, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Esther BaranovJason L Hornick
Jun 20, 2020·Neoplasia : an International Journal for Oncology Research·Ilaria Di MauroFlorence Pedeutour
Jul 22, 2020·Surgical Case Reports·Ken SasakiTakao Ohtsuka
Aug 18, 2020·American Journal of Ophthalmology·Tatyana MilmanRalph C Eagle
Sep 29, 2020·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Lama AlabdulaalySook-Bin Woo
Apr 13, 2021·Clinical Journal of Gastroenterology·Yuko KinowakiMasanobu Kitagawa
Apr 25, 2021·Trends in Cancer·Alvin Qiu, Torsten O Nielsen
Jun 1, 2021·International Journal of Surgical Pathology·Rui PanQiu Rao
Jun 7, 2021·Virchows Archiv : an International Journal of Pathology·Timothy Kwang Yong TayKenneth Tou En Chang
Jun 16, 2021·Diagnostic Pathology·Kentaro MiuraTakeshi Uehara
May 20, 2021·Advances in Anatomic Pathology·Nicholas BaniakMichelle S Hirsch
Jul 20, 2021·Pathology, Research and Practice·Jilong QinPeng Hou
Aug 4, 2021·Indian Journal of Pathology & Microbiology·Bharat Rekhi
May 9, 2021·Histopathology·William J AndersonMichelle S Hirsch
Aug 11, 2021·BMJ Case Reports·Quan DoVikas Singh
Aug 12, 2021·EFORT Open Reviews·Olga SavvidouPanayiotis J Papagelopoulos
May 30, 2021·Advances in Anatomic Pathology·Joon Hyuk Choi, Jae Y Ro
Nov 14, 2020·Surgical Pathology Clinics·Sonja ChenMichael A Arnold
Feb 16, 2021·Ophthalmic Plastic and Reconstructive Surgery·Kalla A GervasioTatyana Milman
Sep 11, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Akihiko YoshidaTaisuke Mori
Sep 20, 2021·Genes, Chromosomes & Cancer·Fleur CordierDavid Creytens
Nov 3, 2021·Cancer Investigation·Neda KhaliliMihan Pourabdollah Toutkaboni