A novel staphylococcal internalization mechanism involves the major autolysin Atl and heat shock cognate protein Hsc70 as host cell receptor

Cellular Microbiology
Nina HirschhausenChristine Heilmann

Abstract

Staphylococcus aureus and Staphylococcus epidermidis can cause serious chronic and recurrent infections that are difficult to eradicate. An important pathogenicity factor in these infections caused by S. aureus is its ability to be internalized by non-professional phagocytes thereby evading the host immune system and antibiotic treatment. Here, we report a novel mechanism involved in staphylococcal internalization by host cells, which is mediated by the major autolysin/adhesins Atl and AtlE from S. aureus and S. epidermidis respectively. In a flow cytometric internalization assay, atl and atlE mutants are significantly reduced in their capacities to be internalized by endothelial cells. Moreover, pre-incubation of endothelial cells with recombinant Atl dose-dependently inhibited internalization. As putative Atl-host cell receptor, the heat shock cognate protein Hsc70 was identified by mass spectrometry. The importance of Hsc70 in internalization was demonstrated by the inhibition of S. aureus internalization with anti-Hsc70 antibodies. In conclusion, this novel Atl- or AtlE-mediated internalization mechanism may represent a 'back-up' mechanism in S. aureus internalization, while it may represent the major or even sole mechanism...Continue Reading

References

Oct 1, 1976·Journal of General Microbiology·S Iordanescu, M Surdeanu
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Oct 1, 1985·Infection and Immunity·S K OgawaF D Lowy
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J EdgellJ B Graham
Jan 1, 1994·Annual Review of Microbiology·J M PattiM Höök
Apr 1, 1993·British Journal of Rheumatology·D De WitK A Davies
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Nov 24, 1998·Infection and Immunity·B E Menzies, I Kourteva
Jan 9, 1999·The New England Journal of Medicine·S BlotF Colardyn
May 27, 1999·Microbial Pathogenesis·J K EllingtonM C Hudson
Mar 18, 2000·International Journal of Antimicrobial Agents·P J McNamara, R A Proctor
Mar 22, 2002·Journal of Virology·Carlos A GuerreroCarlos F Arias

❮ Previous
Next ❯

Citations

Mar 29, 2014·International Journal of Microbiology·Vineet K Singh
Jan 22, 2014·International Journal of Medical Microbiology : IJMM·Friedrich GötzThilo Stehle
Mar 29, 2013·Future Microbiology·Garry LavertyBrendan F Gilmore
Dec 18, 2013·Future Microbiology·Rosanna CoatesMalcolm J Horsburgh
May 16, 2014·BioMed Research International·Nayeli Alva-MurilloAlejandra Ochoa-Zarzosa
Jan 13, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·M ArtiniL Selan
Dec 19, 2015·Ocular Immunology and Inflammation·Ama SadakaMichael S Gilmore
Sep 18, 2015·Journal of Biomedical Materials Research. Part a·Davide CampocciaCarla Renata Arciola
May 21, 2013·International Journal of Medical Microbiology : IJMM·Carsten FehrmannChristine Heilmann
Jan 1, 2016·Trends in Pharmacological Sciences·William E SauseVictor J Torres
Dec 5, 2015·Frontiers in Cellular and Infection Microbiology·Jérôme JosseSophie C Gangloff
Aug 5, 2015·Nucleic Acids Research·Hélène Le PabicBrice Felden
Jul 22, 2014·Letters in Applied Microbiology·P-H ElchingerP Michaud
Sep 14, 2014·Microbiology·Zoran JaglicSusanne Knøchel
Jul 1, 2011·Microbes and Infection·Lucía P AlvarezFernanda R Buzzola
Dec 27, 2011·International Journal of Antimicrobial Agents·Ian M GouldGeorg Peters
Dec 18, 2014·International Journal of Medical Microbiology : IJMM·Muzaffar HussainKarsten Becker
Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Nadzeya KramkoHans-Joachim Schnittler
Nov 19, 2011·FEMS Microbiology Letters·Florian SzabadosSoeren G Gatermann
Oct 4, 2014·Clinical Microbiology Reviews·Karsten BeckerGeorg Peters
Jun 5, 2016·Veterinary Microbiology·Kathleen E BuddOrla M Keane
Jul 10, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M StrobelS Niemann
Dec 21, 2017·Frontiers in Microbiology·Jérôme JosseAlan Diot
Feb 7, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Noriaki YokogawaEdward M Schwarz
Mar 24, 2016·Infection and Immunity·Patrick EbnerFriedrich Götz
Aug 24, 2016·Antimicrobial Agents and Chemotherapy·Tomoyuki HommaBrian P Conlon
Dec 24, 2018·Cellular Microbiology·Adriana Moldovan, Martin J Fraunholz
Mar 20, 2018·Seminars in Immunopathology·Marc G J Feuilloley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.