A Novel Stool PCR Test for Helicobacter pylori May Predict Clarithromycin Resistance and Eradication of Infection at a High Rate

Journal of Clinical Microbiology
Erin BeckmanDino Vaira

Abstract

Clarithromycin-based regimens are commonly used as a first-line therapy for Helicobacter pylori-positive patients; however, resistance to clarithromycin has led to treatment failures. The aim of this study was to evaluate the feasibility of using stool samples to detect the presence of H. pylori DNA while concurrently detecting mutations associated with resistance to clarithromycin. For this purpose, total DNA was extracted from 294 raw stool specimens from H. pylori-positive and -negative patients. TaqMan real-time PCR amplification was used to detect the presence of H. pylori as well as to predict the phenotype of the organism and the related outcome for patients treated with clarithromycin. Clarithromycin resistance was determined upon analysis of the PCR result. Patients were also tested by a urea breath test and were subjected to esophagogastroduodenoscopy, followed by histology, culture, and a rapid urease test, in order to obtain a consensus patient infection status. Of 294 total stool samples, 227 were deemed true positive. The sensitivity of H. pylori detection by PCR was 93.8%. Of 213 true-positive samples that were sequenced, 36.2% showed point mutations associated with clarithromycin resistance (A2142C, A2142G, A214...Continue Reading

References

Aug 15, 1996·FEMS Microbiology Letters·Y J Debets-OssenkoppC M Vandenbroucke-Grauls
Feb 1, 1996·Antimicrobial Agents and Chemotherapy·J VersalovicM F Go
Mar 14, 2001·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·D VairaM Miglioli
Dec 17, 2009·The Journal of Antimicrobial Chemotherapy·Vincenzo De FrancescoDino Vaira
Apr 30, 2010·The New England Journal of Medicine·Kenneth E L McColl
Sep 1, 2015·Gastroenterology Clinics of North America·Francis MégraudPhilippe Lehours
Sep 4, 2015·Molecular and Cellular Probes·Yan Chun ZhuKimberlee A Musser
Dec 24, 2015·Alimentary Pharmacology & Therapeutics·I ThungM A Valasek
Apr 23, 2016·Gastroenterology·Carlo A FalloneJohn K Marshall
Nov 2, 2016·Gut·P MalfertheinerUNKNOWN European Helicobacter and Microbiota Study Group and Consensus panel
Nov 30, 2016·World Journal of Gastroenterology : WJG·Denise E BrennanDeirdre McNamara
Jan 11, 2017·The American Journal of Gastroenterology·William D CheySteven F Moss

❮ Previous
Next ❯

Citations

Sep 12, 2018·Helicobacter·Sabine SkrebinskaEmilie Bessède
Nov 30, 2018·Current Opinion in Gastroenterology·Sinéad M SmithDeirdre McNamara
Dec 21, 2018·Journal of Clinical Microbiology·Toshihiko KakiuchiMuneo Matsuo
Jul 25, 2018·World Journal of Gastroenterology : WJG·Andrea IannoneEnzo Ierardi
Aug 25, 2020·Current Treatment Options in Gastroenterology·Nasir Saleem, Colin W Howden
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes
Jun 12, 2021·Journal of Gastroenterology and Hepatology·Rachel Gingold-BelferDoron Boltin
Aug 16, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Enming QiuShuai Han
Aug 28, 2021·Diagnostics·Hang Yang, Bing Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.