A Novel Strategy for Control of Microbial Biofilms through Generation of Biocide at the Biofilm-Surface Interface.

Applied and Environmental Microbiology
P WoodP Gilbert

Abstract

Biofilms of a mucoid clinical isolate of Pseudomonas aeruginosa (24 h; ca. 10(sup6) CFU/cm(sup2)) were established by immersion of polymer discs in nutrient broth cultures at 37(deg)C. Biofilms exposed for 30 min to various concentrations (0 to 3 mg/ml) of hydrogen peroxide or potassium monopersulfate were rinsed and shaken vigorously in sterile saline to detach loosely associated cells, and the residual viable attached population was quantified by a blot succession method on agar plates. Incorporation of copper and cobalt phthalocyanine catalysts within the polymers significantly enhanced the activity of these oxidizing biocides towards biofilm bacteria by several orders of magnitude. Biofilms established on the control discs resisted treatment with concentrations of either agent of up to 3 mg/ml. Enhancement through incorporation of a catalyst was such that concentrations of potassium monopersulfate of as low as 20 (mu)g/ml gave no recoverable survivors either on the discs or within the washings. Catalysts such as these will promote the formation of active oxygen species from a number of oxidizing agents such as peroxides and persulfates, and it is thought that generation of these at the surface-biofilm interface concentrates...Continue Reading

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Citations

Jun 5, 2010·Journal of Applied Microbiology·R Van Houdt, C W Michiels
Sep 5, 2006·Journal of Environmental Monitoring : JEM·Aine Whelan, Fiona Regan
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·A J McBainP Gilbert
Sep 8, 2006·Applied and Environmental Microbiology·Christine Rabinovitch, Philip S Stewart
Dec 17, 2020·Nanomaterials·Inês B GomesLúcia C Simões
Feb 26, 2019·Antimicrobial Agents and Chemotherapy·Philip S Stewart, Albert E Parker
Mar 13, 2018·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Helen A ThomasonMatthew J Hardman
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Jul 18, 2015·Microbiology Spectrum·Philip S Stewart

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