A novel strategy for the detection and definition of HLA-specific antibodies in patients awaiting renal transplantation

Transplant International : Official Journal of the European Society for Organ Transplantation
J E WorthingtonS Martin

Abstract

Conventional testing for HLA-specific antibodies employs complement-dependent cytotoxicity (CDC) which is labour intensive and dependent on a supply of viable lymphocytes. Our strategy to minimise CDC screening is initially to screen sera by ELISA (Quikscreen) to detect HLA Class I-specific antibodies. Negative sera are then screened by flow cytometry (FCS) using lymphoblastoid cell line pools to detect HLA Class II-specific antibodies. Only Quikscreen- or FCS-positive sera are then tested by CDC and, when indicated, with an ELISA kit (PRA-STAT) for specificity definition. Of 3680 sera, 886 (24.1%) were Quikscreen positive. Of the 2794 Quikscreen-negative sera, 374 (13.4%) were FCS positive. Therefore, only 1265 of the 3680 (34.3%) sera contained HLA-specific antibodies requiring specificity definition. This novel screening strategy has significantly reduced the CDC workload of the laboratory whilst enabling the detection of additional HLA-specific antibodies.

Citations

Jan 31, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Bernadette MageeDerek Middleton
Apr 17, 2012·Pediatric Transplantation·Clifford Chin
Oct 15, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·Byung Ha ChungChul Woo Yang

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