A novel strategy of spine defect repair with a degradable bioactive scaffold preloaded with adipose-derived stromal cells

The Spine Journal : Official Journal of the North American Spine Society
Haixiang LiangFrancis H Shen

Abstract

Although the use of mesenchymal stem cells (MSC) with scaffolds for bone repair has been considered an effective method, the interactions between implanted materials and bone tissues have not been fully elucidated. At some specific sites, such as the vertebral body (VB) of the spine, the process of bone repair with implanted biomaterials is rarely reported. Recently, adipose tissue was found to be an alternative source of MSC besides bone marrow. However, the strategy of using adipose-derived stromal (ADS) cells with bioactive scaffold for the repair of spinal bone defects has seldom been studied. To use a sintered poly(lactide-co-glycolide) acid (PLGA) microspheres scaffold seeded with induced rat ADS cells to repair a bone defect of the VB in a rat model. Basic science and laboratory study. A sintered porous microspheres scaffold was manufactured by PLGA. ADS cells were isolated from Fischer 344 rats and then induced by osteogenic medium with growth and differentiation factor 5 (GDF5) in vitro. Before implantation, cells were cultured with inductive media for 2 weeks as a monolayer situation and 1 more week on a PLGA scaffold as a three-dimensional structure. These assembled bioactive scaffolds then were implanted in lumbar V...Continue Reading

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Citations

Jul 10, 2014·International Orthopaedics·Antonio MarmottiGiuseppe Peretti
Nov 21, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dmitriy SheynDan Gazit
Jan 29, 2016·Journal of Translational Medicine·Amy L StrongBruce A Bunnell
Jul 28, 2015·Advanced Drug Delivery Reviews·Martijn van Griensven
Mar 7, 2021·Bioengineering·André P G Castro
Apr 8, 2021·Biomedical Materials·N Kleer-ReiterA Meyer-Lindenberg

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