A novel strategy to construct yeast Saccharomyces cerevisiae strains for very high gravity fermentation

PloS One
Xianglin TaoXuechang Wu

Abstract

Very high gravity (VHG) fermentation is aimed to considerably increase both the fermentation rate and the ethanol concentration, thereby reducing capital costs and the risk of bacterial contamination. This process results in critical issues, such as adverse stress factors (ie., osmotic pressure and ethanol inhibition) and high concentrations of metabolic byproducts which are difficult to overcome by a single breeding method. In the present paper, a novel strategy that combines metabolic engineering and genome shuffling to circumvent these limitations and improve the bioethanol production performance of Saccharomyces cerevisiae strains under VHG conditions was developed. First, in strain Z5, which performed better than other widely used industrial strains, the gene GPD2 encoding glycerol 3-phosphate dehydrogenase was deleted, resulting in a mutant (Z5ΔGPD2) with a lower glycerol yield and poor ethanol productivity. Second, strain Z5ΔGPD2 was subjected to three rounds of genome shuffling to improve its VHG fermentation performance, and the best performing strain SZ3-1 was obtained. Results showed that strain SZ3-1 not only produced less glycerol, but also increased the ethanol yield by up to 8% compared with the parent strain Z5....Continue Reading

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Citations

Dec 19, 2013·Applied Microbiology and Biotechnology·Dao-Qiong ZhengXue-Chang Wu
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Apr 16, 2014·Food Chemistry·Małgorzata Kus-LiśkiewiczMykhailo Gonchar
Dec 6, 2014·Journal of Industrial Microbiology & Biotechnology·Ke ZhangDaoqiong Zheng
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
Aug 12, 2014·BMC Systems Biology·Ceyda KasaviBetul Kirdar
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Jun 19, 2012·Bioengineered·Cene Gostinčar, Martina Turk
Apr 22, 2017·Applied Microbiology and Biotechnology·Ke ZhangXue-Chang Wu
Nov 18, 2016·Journal of Industrial Microbiology & Biotechnology·Naurú Idalia Vargas-MayaJuan Carlos Torres-Guzmán

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Methods Mentioned

BETA
PCR
electrophoresis
environmental stresses
gene modification
flow cytometry

Software Mentioned

Primer Premier

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