A novel strategy to mimic discontinuous protective epitopes using a synthetic scaffold

Vaccine
Marcel HijnenRob M J Liskamp

Abstract

Although vaccines have been used for a long time and different types of vaccines have been developed, as yet no fully synthetic vaccines have been produced. The production of fully synthetic vaccines has probably not been realized so far due to the structural limitations of linear synthetic peptides to mimic the native shape of protein fragments which is often needed to induce protective antibodies. In this report we used the Bordetella pertussis protein pertactin as a model and show that a novel synthetic scaffold can be used to mimic structurally defined epitopes by confined presentation of several different peptide arms. Guided by modelling a construct was synthesized that induced protective antibodies directed towards a discontinuous epitope. This approach opens up the possibility to the design of new and fully synthetic vaccines that can induce protective antibodies.

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Citations

Apr 9, 2014·Organic Letters·Helmus van de LangemheenRob M J Liskamp
Jan 17, 2012·Journal of Medicinal Chemistry·Christopher J ArnuschYechiel Shai
Jun 8, 2013·Amino Acids·Alessandro GoriGiorgio Colombo
Sep 1, 2012·Chemical Communications : Chem Comm·Gwenn E MulderRob M J Liskamp
Oct 22, 2008·Current Opinion in Chemical Biology·Jutta Eichler
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Dec 29, 2020·Bioorganic Chemistry·Jan HajduchJiří Jiráček

Related Concepts

Bordetella pertussis outer membrane protein P69
Metazoa
Antigenic Specificity
Outer Membrane Lipoproteins, Bacterial
Bordetella pertussis Bacterium
Agglutinogen 2, Bordetella Pertussis
Crystallography, X-Ray
Molecular Mimicry
Mouse, Swiss

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