A novel study on the inhibitory effect of marine macroalgal extracts on hyphal growth and biofilm formation of candidemia isolates.

Scientific Reports
Nessma A El ZawawyMohamed El-Shetehy

Abstract

Biofilm formation and hyphal growth are considered to be the most serious virulence factors of Candida species in blood causing candidemia infections, which are difficult to treat due to the spread of resistant Candida isolates to most antifungal drugs. Therefore, in this study, we investigated the effect of different types and concentrations of selected macroalgal extracts from Cladostephus spongiosus (Phaeophyta), Laurencia papillosa (Rhodophyta), and Codium arabicum (Chlorophyta) in inhibiting those virulence factors of the isolated Candida. Acetone extract of C. spongiosus (AECS) showed a stronger anticandidal activity against the selected strains than ethanol extract. Candida krusei was the highest biofilm producer among the selected isolates. AECS showed an inhibition of C. krusei biofilm formation as well as a reduction in the viability of preformed biofilms. Also, AECS reduced various sugars in the candidal exo-polysaccaride layer (EPS). Scanning electron microscopy (SEM) and light microscopic images revealed an absence of hyphae and an alteration in the morphology of biofilm cells when treated with AECS. Moreover, AECS downregulated the expression of hyphal specific genes, hyphal wall protein 1 (HWP1), Agglutinin-like ...Continue Reading

References

Oct 9, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L BuchailleY Gille
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·G RamageJ L López-Ribot
Oct 31, 2002·Applied and Environmental Microbiology·Gordon RamageJosé L López-Ribot
Jul 23, 2003·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Salvatore De RosaSimeon Popov
Jul 21, 2011·Asian Pacific Journal of Tropical Medicine·Gopalraj Karthikai DeviPerumal Anantharaman
Dec 12, 2012·PloS One·Paul Wai-Kei TsangWing-Ping Fong
Apr 12, 2014·Applied Microbiology and Biotechnology·Raja Mohamed Beema ShafreenKarutha Pandian Shunmugiah
Apr 1, 2016·Scientific Reports·Farazul HaqueMani Shankar Bhattacharyya
May 2, 2017·Journal of Infection in Developing Countries·Nessma Ahmed El Zawawy, ElSayed ElSayed Hafez
Nov 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Suresh Mickymaray, Wael Alturaiki
Oct 18, 2019·Frontiers in Microbiology·Hans CarolusPatrick Van Dijck

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
PCR

Software Mentioned

Light Cycler
SPSS

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.