Abstract
NK cells can kill antibody-coated target cells following engagement of FcgammaRIIIA, the major activating FcgammaR expressed by these cells. The presence of FcgammaRIIC (CD32C) has also been reported, but its contribution to the FcgammaR-dependent effector functions of NK cells remains debated. We demonstrate here that inhibitory FcgammaRIIB is also expressed by a small subset of CD56+/NKp46+ NK cells and can efficiently down-modulate their FcgammaR-dependent effector function. Immunofluorescence analyses of NK cells from 52 healthy donors showed the presence of CD56bright/FcgammaRII(-) (5.2%+/-3.4), CD56dim/FcgammaRII(lo/-) (94.1%+/-3.4), and CD56dim/FcgammaRIIbright (0.64%+/-0.72) cells. QRT-PCR and protein analyses performed on isolated FcgammaRIIbright NK cells indicated that FcgammaRIIB is strongly expressed by these cells but not by FcgammaRII(lo/-) cells. In addition, FcgammaRIIbright cells showed a weaker antibody-dependent degranulation when incubated with IgG-coated target cells compared with FcgammaRII(lo/-) NK cells, although a strong FcgammaRIIIA expression was detected in both cells. Furthermore, the addition of anti-FcgammaRII Fab paralleled a higher degranulation of FcgammaRIIbright NK cells, indicating a direct...Continue Reading
References
Jun 26, 1992·Science·S AmigorenaW H Fridman
Dec 20, 1991·Science·J A OdinJ C Unkeless
May 11, 1990·Science·W Q QiuJ V Ravetch
Jul 1, 1990·The Journal of Experimental Medicine·P A WarmerdamP J Capel
Oct 1, 1989·The Journal of Experimental Medicine·D G BrooksJ V Ravetch
Dec 1, 1987·The Journal of Experimental Medicine·S G StuartK W Moore
Jan 1, 1988·Immunogenetics·L SammartinoO M Garson
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H B FleitJ C Unkeless
Nov 1, 1995·Immunity·M DaëronW H Fridman
Apr 1, 1993·Molecular Immunology·D L CasselS E McKenzie
May 1, 1993·Immunology Today·J G van de Winkel, P J Capel
Jun 8, 1999·European Journal of Immunology·S SivoriA Moretta
Oct 3, 1999·European Journal of Immunology·D MetesP A Morel
Mar 31, 2000·Nature Medicine·R A ClynesJ V Ravetch
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L PricopJ E Salmon
Mar 13, 2001·Annual Review of Immunology·J V Ravetch, S Bolland
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 27, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Linda K ErnstPenelope A Morel
Jul 3, 2004·Cancer Research·Sébastien Dall'OzzoGilles Thibault
Aug 31, 2004·Genes and Immunity·A M Stewart-AkersP A Morel
Dec 18, 2004·Journal of Immunological Methods·Galit AlterMarcus Altfeld
Jan 13, 2006·British Journal of Cancer·L ArnouldB Coudert
Aug 12, 2006·Immunity·Nicolas AnfossiEric Vivier
Aug 23, 2006·The Journal of Experimental Medicine·Meggan MackayBetty Diamond
Sep 11, 2007·Blood·Willemijn B BreunisTaco W Kuijpers
Dec 20, 2007·Cancer Research·Stefania VarchettaFrancesco F Fagnoni
Feb 28, 2008·British Journal of Haematology·Christophe de RomeufHélène Merle-Béral
Citations
Feb 21, 2013·PloS One·Xuan HeYiming Shao
Dec 20, 2013·Science Translational Medicine·Xinrui LiJeffrey C Edberg
Nov 15, 2013·Reviews in Medical Virology·Jop JansGerben Ferwerda
May 20, 2015·Trends in Immunology·Falk NimmerjahnAnja Lux
Jul 12, 2012·Innate Immunity·Yamila S RoccaEstrella M Levy
Aug 8, 2014·European Journal of Immunology·Nupur BhatnagarDirk Meyer-Olson
Jul 20, 2010·Immunological Reviews·Falk Nimmerjahn, Jeffrey V Ravetch
May 19, 2010·Expert Review of Clinical Immunology·Riad AbèsJean-Luc Teillaud
Aug 10, 2011·Annals of the New York Academy of Sciences·André J NahmiasCarlos Abramowsky
Sep 8, 2019·Medical Oncology·Feng CuiKejun Nan
Jul 17, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manuela MoraruCarlos Vilches
Oct 27, 2015·Immunological Reviews·Pierre Bruhns, Friederike Jönsson
Oct 27, 2015·Immunological Reviews·Sina GordanFalk Nimmerjahn
Mar 15, 2018·Frontiers in Immunology·Daniëlle KrijgsmanPeter J K Kuppen
Oct 23, 2019·Disease Markers·Feng CuiYuqing Xu
May 14, 2020·Cancer Immunology, Immunotherapy : CII·Daniëlle KrijgsmanMarianne Hokland
May 6, 2019·Cancer Immunology, Immunotherapy : CII·Daniëlle KrijgsmanPeter J K Kuppen
Mar 2, 2021·Frontiers in Immunology·Christian BinderDavid Berglund
Jan 8, 2021·Journal of Translational Autoimmunity·Seung Y ChuWilliam Stohl
Nov 4, 2020·Molecular Immunology·Daniëlle KrijgsmanPeter J K Kuppen
Dec 24, 2011·Biochimica Et Biophysica Acta·Li-Hua WangHe-Ping Ma