A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function

Journal of Leukocyte Biology
Charles-Antoine DutertreJean-Luc Teillaud

Abstract

NK cells can kill antibody-coated target cells following engagement of FcgammaRIIIA, the major activating FcgammaR expressed by these cells. The presence of FcgammaRIIC (CD32C) has also been reported, but its contribution to the FcgammaR-dependent effector functions of NK cells remains debated. We demonstrate here that inhibitory FcgammaRIIB is also expressed by a small subset of CD56+/NKp46+ NK cells and can efficiently down-modulate their FcgammaR-dependent effector function. Immunofluorescence analyses of NK cells from 52 healthy donors showed the presence of CD56bright/FcgammaRII(-) (5.2%+/-3.4), CD56dim/FcgammaRII(lo/-) (94.1%+/-3.4), and CD56dim/FcgammaRIIbright (0.64%+/-0.72) cells. QRT-PCR and protein analyses performed on isolated FcgammaRIIbright NK cells indicated that FcgammaRIIB is strongly expressed by these cells but not by FcgammaRII(lo/-) cells. In addition, FcgammaRIIbright cells showed a weaker antibody-dependent degranulation when incubated with IgG-coated target cells compared with FcgammaRII(lo/-) NK cells, although a strong FcgammaRIIIA expression was detected in both cells. Furthermore, the addition of anti-FcgammaRII Fab paralleled a higher degranulation of FcgammaRIIbright NK cells, indicating a direct...Continue Reading

References

Jul 1, 1990·The Journal of Experimental Medicine·P A WarmerdamP J Capel
Dec 1, 1987·The Journal of Experimental Medicine·S G StuartK W Moore
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H B FleitJ C Unkeless
May 1, 1993·Immunology Today·J G van de Winkel, P J Capel
Mar 31, 2000·Nature Medicine·R A ClynesJ V Ravetch
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L PricopJ E Salmon
Mar 13, 2001·Annual Review of Immunology·J V Ravetch, S Bolland
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 27, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Linda K ErnstPenelope A Morel
Aug 31, 2004·Genes and Immunity·A M Stewart-AkersP A Morel
Dec 18, 2004·Journal of Immunological Methods·Galit AlterMarcus Altfeld
Aug 12, 2006·Immunity·Nicolas AnfossiEric Vivier
Aug 23, 2006·The Journal of Experimental Medicine·Meggan MackayBetty Diamond

❮ Previous
Next ❯

Citations

Dec 20, 2013·Science Translational Medicine·Xinrui LiJeffrey C Edberg
Nov 15, 2013·Reviews in Medical Virology·Jop JansGerben Ferwerda
May 20, 2015·Trends in Immunology·Falk NimmerjahnAnja Lux
Aug 8, 2014·European Journal of Immunology·Nupur BhatnagarDirk Meyer-Olson
Jul 20, 2010·Immunological Reviews·Falk Nimmerjahn, Jeffrey V Ravetch
May 19, 2010·Expert Review of Clinical Immunology·Riad AbèsJean-Luc Teillaud
Oct 27, 2015·Immunological Reviews·Pierre Bruhns, Friederike Jönsson
Mar 15, 2018·Frontiers in Immunology·Daniëlle KrijgsmanPeter J K Kuppen
May 14, 2020·Cancer Immunology, Immunotherapy : CII·Daniëlle KrijgsmanMarianne Hokland
Mar 2, 2021·Frontiers in Immunology·Christian BinderDavid Berglund
Dec 24, 2011·Biochimica Et Biophysica Acta·Li-Hua WangHe-Ping Ma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.