A novel surface acoustic wave-based biosensor for highly sensitive functional assays of olfactory receptors

Biochemical and Biophysical Research Communications
Chunsheng WuPing Wang

Abstract

Olfactory receptors, which are responsible for sensing odor molecules, form the largest G protein-coupled receptor (GPCR) family in mammalian animals. These proteins play an important role in the detection of chemical signals and signal transduction to the brain. Currently, only a limited number of olfactory receptors have been characterized, which is mainly due to the lack of sensitive and efficient tools for performing functional assays of these receptors. This paper describes a novel surface acoustic wave (SAW)-based biosensor for highly sensitive functional assays of olfactory receptors. An olfactory receptor of Caenorhabditis elegans, ODR-10, was expressed on the plasma membrane of human breast cancer MCF-7 cells, which was used as a model system for this study. For specific odorant response assays, the membrane fraction of MCF-7 cells containing ODR-10 was extracted and integrated with our SAW sensors. The response of ODR-10 to various odorants was monitored by recording the resonance frequency shifts of SAWs applied to the sensor. Our results show that heterologously expressed ODR-10 receptors can specifically respond to diacetyl, its natural ligand. Dose-dependent responses were obtained by performing measurements using...Continue Reading

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Citations

Dec 25, 2012·Biosensors & Bioelectronics·Liping DuPing Wang
Apr 12, 2013·Future Medicinal Chemistry·Kenneth Lundstrom
Aug 28, 2012·Trends in Biotechnology·Sofia A M MartinsD Miguel F Prazeres
Jun 22, 2012·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·I YahyaieD R Oliver
Oct 6, 2012·Biosensors & Bioelectronics·Liping DuPing Wang
Jul 15, 2015·Bioinspiration & Biomimetics·S B OlssonT C Pearce
Feb 14, 2021·Biosensors & Bioelectronics·Yuji SukekawaTakamichi Nakamoto
Jun 17, 2021·Lab on a Chip·Yusuke HirataShoji Takeuchi

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