A novel technique for quantitative detection of mRNA expression in human bone derived cells cultured on biomaterials

Journal of Biomedical Materials Research
H ZreiqatC R Howlett

Abstract

A nonisotopic and quantitative in situ hybridization technique was adapted to investigate the effect of biomaterials on the cellular expression of mRNA from human bone derived cells (HBD cells). HBD cells were cultured for 24 or 48 h on tissue culture plastic, alumina, and ion modified alumina. Osteocalcin, osteopontin, alkaline phosphatase, type I collagen alpha 1, and type I collagen alpha 2 mRNAs were quantified. Protein expression for collagen types I, III, and V, and for anti-human macrophages CD68 (DAKO-CD68, KP1) and CD68 (PG-M1), and anti-human myeloid/histiocyte antigen (DAKO-MAC 387) were determined immunohistochemically using monoclonal antibodies. At 24 and 48 h, levels of mRNA for alkaline phosphatase and osteonectin were greater than mRNA levels for osteopontin, osteocalcin, collagen type I alpha 1, and collagen type I alpha 2 for cells grown on the three substrata. However, at 48 h mRNA levels for alkaline phosphatase and osteonectin were significantly higher on the modified ceramic substrata relative to the native alumina. HBD cells appear to express CD68-KP1 when cultured for 24 h. The techniques provide a sensitive and reproducible assay to evaluate gene and protein expression of cells grown on different subst...Continue Reading

References

Apr 1, 1991·Genomics·N C DracopoliS K Mishra
Jan 1, 1990·International Immunology·K A PulfordD Y Mason
Apr 25, 1989·Nucleic Acids Research·M C KieferP J Barr
Sep 1, 1985·Calcified Tissue International·P G Robey, J D Termine
May 15, 1988·Analytical Biochemistry·M Wilchek, E A Bayer
Mar 1, 1986·Calcified Tissue International·C G BellowsM E Antosz
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C MyersF Ramirez
Sep 1, 1985·Calcified Tissue International·Pamela Gehron Robey, John D Termine

❮ Previous
Next ❯

Citations

Feb 24, 2001·Journal of Biomedical Materials Research·T G van KootenC J Kirkpatrick
Sep 5, 2002·Journal of Biomedical Materials Research·H ZreiqatM Shakibaei
Dec 17, 2002·Journal of Biomedical Materials Research. Part a·H ZreiqatL Hanley
Jul 20, 2007·Journal of Molecular Histology·H ZreiqatC L Geczy
Jul 20, 2007·Journal of Biomedical Materials Research. Part a·C KnabeM Stiller
Jan 12, 2005·Clinical Oral Implants Research·Christine KnabeHala Zreiqat
Mar 4, 2006·Journal of Biomedical Materials Research. Part a·Galateia J KazakiaTony M Keaveny
Mar 26, 1999·Journal of Biomaterials Science. Polymer Edition·H ZreiqatC R Howlett
Sep 17, 1999·Journal of Biomedical Materials Research·H Zreiqat, C R Howlett
Mar 16, 2000·Journal of Biomaterials Science. Polymer Edition·A HiguchiM Hara
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·Christine KnabeHala Zreiqat
Jul 9, 1999·Journal of Biomedical Materials Research·H ZreiqatC R Howlett
Dec 22, 1999·Journal of Biomedical Materials Research·L BacákováV Svorcík
May 13, 1999·Journal of Biomedical Materials Research·C R HowlettD R McKenzie
Jun 9, 2006·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C KnabeM Stiller
Sep 13, 2001·Journal of Biomedical Materials Research·F A AkinL Hanley
Jun 5, 2008·Journal of Biomedical Materials Research. Part a·Chaoyuan WangHala Zreiqat
Jul 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Subhash SistaGopal Pande

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.