A novel technique that measures peptide secretion on a millisecond timescale reveals rapid changes in release

Neuron
Matthew D Whim, Guy W J Moss

Abstract

Neuropeptides are ubiquitous transmitters that have been implicated in a wide variety of physiological and pathological conditions, and it is important to understand the processes that control their secretion. We have developed a technique that measures neuropeptide secretion with high temporal resolution. This method involves placing an electrophysiological "tag" in a neuropeptide prohormone. The tagged prohormone is subsequently expressed together with an ionotropic receptor that binds the tag. Because the neuropeptide of interest and the tag enter the same population of dense core granules, neuropeptide secretion gives rise to fast, synaptic-like currents. Using this method, we show that peptide secretion can be modulated on a millisecond time scale. This technique could be readily adapted to measure the secretion of any neuropeptide.

References

May 1, 1979·The Journal of Physiology·A Dutton, R E Dyball
Aug 12, 1977·Science·D A Price, M J Greenberg
Jul 1, 1991·Physiological Reviews·I Kupfermann
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M D Whim, P E Lloyd
Feb 1, 1988·The Journal of Cell Biology·L MatsuuchiR B Kelly
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H Y YangE Costa
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C D MinthJ E Dixon
Oct 1, 1994·Pflügers Archiv : European journal of physiology·K A GreenG A Cottrell
Jun 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J BeanT Hökfelt
Apr 1, 1994·The Biochemical Journal·P A Halban, J C Irminger
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·C Wahlestedt, D J Reis
May 1, 1997·Current Biology : CB·R A Chavez, H P Moore
Jun 1, 1997·The Journal of General Physiology·C M McNicholas, C M Canessa
Jul 1, 1997·Trends in Neurosciences·A G Blomqvist, H Herzog
May 9, 1998·The Journal of Biological Chemistry·S CoscoyP Barbry
Jul 8, 1999·Current Opinion in Neurobiology·R S Zucker
May 20, 2000·Neuropharmacology·T HökfeltM Diez

❮ Previous
Next ❯

Citations

Jun 9, 2004·Methods : a Companion to Methods in Enzymology·Matthew D Whim, Guy W J Moss
Jun 6, 2003·Progress in Neurobiology·Volkmar LessmannMarzia Malcangio
May 5, 2012·Nature Protocols·Rayner Rodriguez-DiazAlejandro Caicedo
Feb 11, 2004·The Journal of General Physiology·Matthias BraunPatrik Rorsman
May 1, 2009·Journal of Neurophysiology·Hajime HirasawaElio Raviola
Jan 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gregory C MitchellMatthew D Whim
Aug 2, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qian WangMatthew D Whim
Dec 19, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Prabhu Ramamoorthy, Matthew D Whim
Jan 17, 2015·Cell·George A LemieuxKaveh Ashrafi
Oct 9, 2012·Neuron·Anthony N van den Pol
Jun 16, 2009·Neuroscience Research·Volkmar Lessmann, Tanja Brigadski
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Frank Thévenod
Aug 28, 2020·Frontiers in Cellular Neuroscience·Bing ChenXin-Peng Dun
Feb 14, 2018·The Journal of Experimental Biology·Kellen DeLaneyLingjun Li
Oct 27, 2009·Progress in Neurobiology·Xu ZhangGuo-Qiang Ma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.