A novel tetravalent formulation combining the four aggregated domain III-capsid proteins from dengue viruses induces a functional immune response in mice and monkeys

International Immunology
Edith SuzarteLisset Hermida

Abstract

Our group developed a subunit vaccine candidate against dengue virus based on two different viral regions: the domain III of the envelope protein and the capsid protein. The novel chimeric protein from dengue-2 virus [domain III-capsid (DIIIC-2)], when presented as aggregated incorporating oligodeoxynucleotides, induced anti-viral and neutralizing antibodies, a cellular immune response and conferred significant protection to mice and monkeys. The remaining constructs were already obtained and properly characterized. Based on this evidence, this work was aimed at assessing the immune response in mice of the chimeric proteins DIIIC of each serotype, as monovalent and tetravalent formulations. Here, we demonstrated the immunogenicity of each protein in terms of humoral and cell-mediated immunity, without antigen competition on the mixture forming the formulation tetra DIIIC. Accordingly, significant protection was afforded as measured by the limited viral load in the mouse encephalitis model. The assessment of the tetravalent formulation in non-human primates was also conducted. In this animal model, it was demonstrated that the formulation induced neutralizing antibodies and memory cell-mediated immune response with IFN-γ-secreti...Continue Reading

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Citations

Feb 23, 2018·Applied Microbiology and Biotechnology·Hossein FahimiMajid Sadeghizadeh
Jan 25, 2019·Expert Review of Vaccines·Laura LazoLázaro Gil
Aug 19, 2016·Nature Reviews. Disease Primers·Maria G GuzmanScott B Halstead
Apr 25, 2017·Clinical & Translational Immunology·Laura Lazo VázquezLisset Hermida Cruz
Sep 8, 2018·Frontiers in Immunology·Nagesh K Tripathi, Ambuj Shrivastava
Aug 28, 2020·Expert Review of Vaccines·Laura Lazo

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