A novel thermophilic Aeribacillus bacteriophage AP45 isolated from the Valley of Geysers, Kamchatka: genome analysis suggests the existence of a new genus within the Siphoviridae family

Extremophiles : Life Under Extreme Conditions
V MorozovaN Tikunova

Abstract

A novel thermophilic bacteriophage AP45 and its host strain Aeribacillus sp. CEMTC656 were isolated from the Valley of Geysers, Kamchatka Peninsula, Russia. Bacteriophage AP45 was identified as a member of the Siphoviridae family by electron microscopy. It showed high thermostability and had a slow cycle of reproduction. The AP45 genome had 51,606 base pairs (bp) and contained 71 open reading frames (ORFs), 40 of them encoding proteins of predicted function. Genes encoding DNA and RNA polymerases were not identified, indicating that AP45 used host polymerases. Based on the ORF65 encoding putative endolysin, the recombinant protein rAP45Lys was developed and its peptidoglycan-hydrolyzing activity was demonstrated. The AP45 genome exhibited limited identity to other phage sequences; the highest identity, 36%, was with the genome of the thermophilic Geobacillus myovirus D6E. The majority of putative proteins encoded by the AP45 genome had higher similarity to proteins from bacteria belonging to the Bacillaceae family, than to bacteriophages. In addition, more than half of the putative ORFs in the AP45 genome were highly similar to prophage sequences of A. pallidus strain 8m3, which was isolated in north-east China. The AP45 phage ...Continue Reading

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Citations

Nov 25, 2020·BMC Microbiology·Yuriy L OrlovOxana B Dobrovolskaya
Aug 8, 2021·Microorganisms·Beata ŁubkowskaPiotr M Skowron

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Datasets Mentioned

BETA
MF346725
KX965989
KCTC3564

Methods Mentioned

BETA
light microscopy
phage purification
electron microscopy
PCR
electrophoresis

Software Mentioned

InterProScan
SignalP
MEGA
BLAST
DELTA
MAFFT
BioEdit
BLASTX
PHAST
HHPred

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