A novel thermostable and organic solvent-tolerant lipase from Xanthomonas oryzae pv. oryzae YB103: screening, purification and characterization

Extremophiles : Life Under Extreme Conditions
Qiurun MoMu Li

Abstract

Thermostable lipases offer major biotechnological advantages over mesophilic lipases. In this study, an intracellular thermostable and organic solvent-tolerant lipase-producing strain YB103 was isolated from soil samples and identified taxonomically as Xanthomonas oryzae pv. oryzae. The lipase from X. oryzae pv. oryzae YB103 (LipXO) was purified 101.1-fold to homogeneity with a specific activity of 373.9 U/mg. The purified lipase showed excellent thermostability, exhibiting 51.1% of its residual activity after incubation for 3 days at 70 °C. The enzyme showed optimal activity at 70 °C, suggesting it is a thermostable lipase. LipXO retained 75.1-154.1% of its original activity after incubation in 20% (v/v) hydrophobic organic solvents at 70 °C for 24 h. Furthermore, LipXO displayed excellent stereoselectivity (e.e.p >99%) toward (S)-1-phenethyl alcohol in n-hexane. These unique properties of LipXO make it promising as a biocatalyst for industrial processes.

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Citations

Feb 21, 2018·Biotechnology Journal·Marina LottiPau Ferrer
Dec 12, 2018·Biotechnology Letters·Priyanka PriyankaBarry J Ryan
Dec 3, 2016·Frontiers in Microbiology·Dimitra ZarafetaGeorgios Skretas
Oct 1, 2020·Journal of Food Biochemistry·Taiwo O AkanbiDominic Agyei
Sep 7, 2021·Applied Microbiology and Biotechnology·Siti Hajar HamdanThean Chor Leow

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