PMID: 9540792Apr 16, 1998Paper

A novel thermostable neutral proteinase from Saccharomonospora canescens

Biochimica Et Biophysica Acta
P DolashkaW Voelter

Abstract

A novel thermostable neutral proteinase, called NPS, was purified to electrophoretic homogeneity from the culture broth of Saccharomonospora canescens sp. novus, strain 5. The molecular mass was determined by SDS-polyacrylamide gel electrophoresis to be 35,000 Da. The enzyme exhibits a sharp pH optimum of proteolytic activity at pH 6.7. NPS was completely inactivated with inhibitors, typical for metalloendopeptidases, EDTA and 1,10-phenantroline, whereas the serine proteinase inhibitor PMSF had no effect. Atomic absorption measurements showed that the proteinase binds a single zinc and four calcium ions. The enzyme thermostability was characterized in the absence and presence of added calcium. Melting temperature, Tm = 77 degrees C and an activation energy, Ea, for the thermal deactivation of the excited protein fluorophores of 72.13 kJ mol-1 were calculated in the presence of 100 mM CaCl2. The Ea-value is considerably higher than those obtained for a number of proteinases from microorganisms and was explained by the thermostable structure of the enzyme. Effective radiationless energy transfer from phenol groups to indole rings was observed. 68% of the light absorbed by tyrosyl residues is transferred to tryptophyl side chains....Continue Reading

References

Jan 13, 1976·Biochemistry·B Holmquist, B L Vallee
Sep 1, 1977·Bacteriological Reviews·F G Priest
Jun 1, 1990·Trends in Pharmacological Sciences·B P Roques, A Beaumont
Mar 21, 1967·Biochemical and Biophysical Research Communications·K Morihara
Mar 11, 1968·Archives of Biochemistry and Biophysics·K MoriharaT Oka
Mar 1, 1980·Journal of Bacteriology·L K JolliffeU N Streips
Jan 1, 1983·Molecular and Cellular Biochemistry·M Philipp, M L Bender
Sep 27, 1983·Biochemistry·P A Bartlett, C K Marlowe
Sep 16, 1981·Biochemical and Biophysical Research Communications·J AlmenoffM Orlowski
Oct 5, 1982·Journal of Molecular Biology·M A Holmes, B W Matthews
Sep 23, 1994·Journal of Molecular Biology·U Baumann
Jul 16, 1962·Biochimica Et Biophysica Acta·J A GALLY, G M EDELMAN
Jul 12, 1972·Nature: New Biology·K TitaniH Neurath
Mar 20, 1935·The Journal of General Physiology·M Kunitz

❮ Previous
Next ❯

Citations

Jan 22, 2009·Applied Biochemistry and Biotechnology·Vadde RamakrishnaLokireddy Sudarsana Reddy
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P Dolashka-AngelovaW Voelter
Apr 20, 2010·International Journal of Systematic and Evolutionary Microbiology·Jun MaoZhi-Heng Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.