A novel thermostable xylanase from Thermomonospora sp.: influence of additives on thermostability

Bioresource Technology
S P GeorgeM B Rao

Abstract

An alkalothermophilic Thermomonospora sp. producing high levels of xylanase was isolated from self-heating compost. The culture produced 125 IU/ml of xylanase when grown in shake flasks at pH 9 and 50 degrees C for 96 h. The culture filtrate also contained cellulase (23 IU/ml), mannanase (1 IU/ml) and beta-xylosidase (0.1 IU/ml) activities. The xylanase was active at a broad range of pH (5-9) and temperature (40-90 degrees C). The optimum pH and temperature were 7 and 70 degrees C, respectively. The enzyme was stable in the pH range 5-8 and was thermostable with half-lives of 8 and 4 h at 60 degrees C and 70 degrees C, respectively, but only 9 min at 80 degrees C. The effects of a variety of compounds to enhance the stability of xylanase at 80 degrees C was studied. Addition of sorbitol, mannitol and glycerol increased the thermostability of xylanase in proportion to the number of hydroxyl groups per polyol molecule. Glycine also offered protection against thermoinactivation. Xylan, trehalose, gelatin and trehalose-gelatin mixture had marginal effect on the thermostability of xylanase at 80 degrees C.

References

Nov 27, 1991·Biochemical and Biophysical Research Communications·R A HallewellG T Mullenbach
Jan 1, 1969·European Journal of Biochemistry·H Kersters-HildersonC K De Bruyne
Jul 28, 1999·FEMS Microbiology Reviews·N KulkarniM Rao
Mar 29, 2001·Bioresource Technology·S P GeorgeM B Rao

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Citations

Dec 17, 2008·Journal of Industrial Microbiology & Biotechnology·P ShahJayant Malhar Khire
Sep 21, 2010·Applied and Environmental Microbiology·Ravi D BaraboteAlison M Berry
Mar 13, 2014·World Journal of Microbiology & Biotechnology·Wei WangChunyu Yang
Feb 24, 2016·Enzyme Research·Hemlata BhosaleTukaram Kadam
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·B BouizgarneY Ouhdouch
Feb 18, 2005·Journal of Biochemistry and Molecular Biology·Asano KrisanaKirtikara Kanyawim
Dec 3, 2014·Functional & Integrative Genomics·Haiwei FengPei Zhou
Apr 8, 2017·BioMed Research International·Ulfuara ShefaYoungbuhm Huh
Feb 18, 2005·Journal of Biochemistry and Molecular Biology·Isaac Yi KimSeong-Jin Kim
May 31, 2018·Iranian Journal of Biotechnology·Karuppasamy Geetha, Paramasamy Gunasekaran
Dec 15, 2020·International Journal of Biological Macromolecules·Umber ZamanAnwar Iqbal
Mar 25, 2019·International Journal of Biological Macromolecules·Gabriela Salvador de AmoGustavo Orlando Bonilla-Rodriguez

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