A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

Biotechnology for Biofuels
Liliane Fc RibeiroMaria de Lourdes Tm Polizeli

Abstract

The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. Xylanase MpXyn10A was overexpressed in Aspergillus nidulans and was active against birchwood xylan, presenting an optimum activity at pH 5.8 and 80°C. MpXyn10A was 16% glycosylated and thermostable, preserving 85% activity after 24 hours at 65°C, and deglycosylation did not affect thermostability. Circular dichroism confirmed the high alpha-helical content consistent with the canonical GH10 family (β/α)8 barrel fold observed in molecular modeling. Primary structure analysis revealed the existence of eight cysteine residues which could be involved in four disulfide bonds, and this could explain the high thermostability of this enzyme even in the deglycosylated form. MpXyn10A showed promising results in biomass degradation, increasing the amount of reducing sugars in bagasse in natura and ...Continue Reading

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Citations

Apr 27, 2016·Applied Microbiology and Biotechnology·L R S Moreira, E X F Filho
Jan 19, 2018·Critical Reviews in Biotechnology·Abdul BasitHuiqiang Lou
Jun 6, 2018·Frontiers in Plant Science·Chunlei YuCaixia Wang
Apr 26, 2020·Scientific Reports·Lummy Maria Oliveira MonteiroMaria de Lourdes Teixeira de Moraes Polizeli
Oct 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana Sílvia de Almeida ScarcellaMaria de Lourdes Teixeira de Moraes Polizeli
Oct 24, 2019·Applied Microbiology and Biotechnology· Alokika, Bijender Singh
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Zhaoxing LiuNing Hao
Apr 24, 2018·International Journal of Biological Macromolecules·Sunny SharmaBijender Kumar Bajaj
May 14, 2021·Biotechnology Reports·Lummy Maria Oliveira MonteiroMaria de Lourdes Teixeira de Moraes Polizeli

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Datasets Mentioned

BETA
KJ767674

Methods Mentioned

BETA
glycosylation
PCR
electrophoresis
size exclusion chromatography
circular dichroism
acetylation
enzymatic assay

Software Mentioned

BLAST ( Iterated Basic Local Alignment Search Tool )
MODELLER9v12
MODELLER
PSI
SigrafW
MATLAB
MTSA46
PROCHECK
NetNGlyc1
NetOGlyc3

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