Abstract
The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. Xylanase MpXyn10A was overexpressed in Aspergillus nidulans and was active against birchwood xylan, presenting an optimum activity at pH 5.8 and 80°C. MpXyn10A was 16% glycosylated and thermostable, preserving 85% activity after 24 hours at 65°C, and deglycosylation did not affect thermostability. Circular dichroism confirmed the high alpha-helical content consistent with the canonical GH10 family (β/α)8 barrel fold observed in molecular modeling. Primary structure analysis revealed the existence of eight cysteine residues which could be involved in four disulfide bonds, and this could explain the high thermostability of this enzyme even in the deglycosylated form. MpXyn10A showed promising results in biomass degradation, increasing the amount of reducing sugars in bagasse in natura and ...Continue Reading
References
Jul 1, 1965·Genetics·R W BarrattW N Ogata
Dec 1, 1983·Gene·J TilburnR W Davies
Nov 1, 1995·Proteins·A SaliM Karplus
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 6, 1998·European Journal of Biochemistry·M L MattinenA Annila
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·R MaheshwariM K Bhat
Jul 5, 2003·Analytical Biochemistry·Vincent RaussensErik Goormaghtigh
Apr 1, 2004·Applied Biochemistry and Biotechnology·Mônica Caramez Triches DamasoNei Pereira
Mar 10, 2007·The Plant Journal : for Cell and Molecular Biology·Radnaa NaranAndrew J Mort
Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson
Apr 19, 2008·Applied Biochemistry and Biotechnology·Michael J SeligWilliam S Adney
Aug 1, 2008·Applied Biochemistry and Biotechnology·Ines MaalejHafedh Belghith
Nov 13, 2010·Biochemical and Biophysical Research Communications·Camila Ramos SantosMario Tyago Murakami
Apr 7, 2011·Microbial Cell Factories·Mickael LafondThierry Giardina
Nov 1, 2005·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Francisco Assis LeoneIvana Aparecida Borin
Nov 10, 2011·The Journal of Microbiology·Marita Gimenez PereiraJoão Atílio Jorge
Jun 6, 2012·Enzyme and Microbial Technology·Fernando SegatoRolf A Prade
Jul 4, 2012·Acta Crystallographica. Section D, Biological Crystallography·Maria DimarogonaEvangelia D Chrysina
Sep 11, 2012·Advances in Applied Microbiology·Xiaoyun SuIsaac K O Cann
Mar 29, 2013·Applied Biochemistry and Biotechnology·Peng HanZhengqiang Jiang
Apr 2, 2013·Journal of the American Chemical Society·Glyn R HemsworthPaul H Walton
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·T A GorshkovaP V Mikshina
Jan 1, 1994·Bioscience, Biotechnology, and Biochemistry·S NakamuraK Horikoshi
Citations
Jan 19, 2016·Food Chemistry·Xiaoyu WangBin Yao
Aug 1, 2015·Scientific Reports·Hanpeng LiaoYangchun Xu
Jul 2, 2015·Journal of Agricultural and Food Chemistry·Xiongliang HuangShuang Li
May 23, 2015·Biotechnology for Biofuels·Hiroyuki InoueKazuhiko Ishikawa
Apr 27, 2016·Applied Microbiology and Biotechnology·L R S Moreira, E X F Filho
Oct 30, 2016·Bioresource Technology·Xiaoyu WangHuiying Luo
Mar 23, 2018·AMB Express·Youzhi MiaoRuifu Zhang
Jan 19, 2018·Critical Reviews in Biotechnology·Abdul BasitHuiqiang Lou
Nov 22, 2017·Biotechnology for Biofuels·Silvia HüttnerLisbeth Olsson
Feb 15, 2018·BMC Biotechnology·Anil S PrajapatiR B Subramanian
Oct 17, 2019·Journal of Biochemistry·Rasha A M AzouzAfaf S Fahmy
Jun 6, 2018·Frontiers in Plant Science·Chunlei YuCaixia Wang
Jun 23, 2018·Biotechnology for Biofuels·Shuai YouBin Yao
Mar 5, 2020·Biotechnology for Biofuels·Chao HanDuochuan Li
Apr 26, 2020·Scientific Reports·Lummy Maria Oliveira MonteiroMaria de Lourdes Teixeira de Moraes Polizeli
Oct 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana Sílvia de Almeida ScarcellaMaria de Lourdes Teixeira de Moraes Polizeli
Apr 7, 2020·Applied Microbiology and Biotechnology·Namrata JoshiSudhir P Singh
Feb 12, 2017·PloS One·Xiaoyu ChangBin Yao
Jun 26, 2018·Applied Biochemistry and Biotechnology·Valentina Echeverría, Jaime Eyzaguirre
Dec 19, 2017·PloS One·Xiaoyu WangHuiying Luo
Oct 24, 2019·Applied Microbiology and Biotechnology· Alokika, Bijender Singh
Mar 9, 2021·Frontiers in Bioengineering and Biotechnology·Zhaoxing LiuNing Hao
Apr 4, 2021·Microorganisms·Alex Graça ContatoMaria de Lourdes Teixeira de Moraes Polizeli
Apr 24, 2018·International Journal of Biological Macromolecules·Sunny SharmaBijender Kumar Bajaj
May 14, 2021·Biotechnology Reports·Lummy Maria Oliveira MonteiroMaria de Lourdes Teixeira de Moraes Polizeli
Aug 8, 2021·Microorganisms·Dung Minh Ha-TranChieh-Chen Huang