A Novel Thioredoxin-Dependent Peroxiredoxin (TPx-Q) Plays an Important Role in Defense Against Oxidative Stress and Is a Possible Drug Target in Babesia microti

Frontiers in Veterinary Science
Houshuang ZhangJinlin Zhou

Abstract

Thioredoxin peroxidases (TPxs) are ubiquitous cysteine-based peroxidases that reduce peroxides as part of antioxidant defenses and redox signaling and are essential for Babesia microti protection against adverse environment agents like reactive oxygen species (ROS) and reactive nitrogen species (RNS). To better systematically understand TPxs, we identified a novel 2-Cys peroxiredoxin-Q (BmTPx-Q) of B. microti. The full-length BmTPx-Q gene is 653 bp that consists of an intact open reading frame of 594 bp that encodes a 197-amino acid protein. The predicted protein has a molecular weight of 22.3 kDa and an isoelectric point of 9.18. Moreover, BmTPx-Q showed low identity at the amino acid level to other peroxiredoxins (Prxs) among the currently known subfamilies. The recombinant BmTPx-Q protein (rBmTPx-Q) was expressed in Escherichia coli and purified with beads. The native protein BmTPx-Q was detected using mouse anti-BmTPx-Q polyclonal serum with western blotting and indirect immunofluorescence assay (IFA). In addition, enzyme activity was observed using nicotinamide adenine dinucleotide phosphate (NADPH) as substrate and triggered the NADPH-dependent reduction of the Trx/TrxR system. It was also discovered that BmTPx-Q mainly e...Continue Reading

References

Feb 1, 1972·European Journal of Biochemistry·R G ThurmanR Scholz
Mar 28, 1995·Biochemical and Biophysical Research Communications·H SauriH Shau
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Z ChaeS G Rhee
Apr 16, 1998·The Journal of Biological Chemistry·S W KangS G Rhee
Jul 25, 2000·Clinical Microbiology Reviews·M J HomerD H Persing
May 11, 2002·The Journal of Biological Chemistry·Zita KrnajskiSylke Müller
Aug 22, 2002·Redox Report : Communications in Free Radical Research·Junichi Fujii, Yoshitaka Ikeda
Sep 12, 2003·Emerging Infectious Diseases·Barbara L HerwaldtNorman J Pieniazek
Jun 9, 2004·Scandinavian Journal of Immunology·K FredrikssonC M Skold
Feb 11, 2005·Journal of Molecular Biology·Ganapathy N SarmaP Andrew Karplus
Feb 16, 2005·Parasitology International·Kazuhiko YanoShin-ichiro Kawazu
May 14, 2005·Free Radical Biology & Medicine·Nicolas Rouhier, Jean-Pierre Jacquot
Nov 26, 2005·Biological Chemistry·Christine NickelKatja Becker
Mar 1, 2006·Veterinary Parasitology·Henri J Vial, A Gorenflot
Sep 25, 2007·Parasitology International·Shin-ichiro KawazuShigeyuki Kano
May 30, 2009·The FEBS Journal·Andrea HallLeslie B Poole
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sasa KoncarevicKatja Becker
May 27, 2010·Parasite Immunology·M W RobinsonS Donnelly
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal
Jan 5, 2011·Antioxidants & Redox Signaling·Karl-Josef Dietz
Mar 1, 2010·Ticks and Tick-borne Diseases·Jeremy GrayLouis Weiss
Aug 6, 2011·Current Protocols in Toxicology·Leslie B PooleKimberly J Nelson
Mar 7, 2012·Parasitology Research·Hassan HakimiShin-Ichiro Kawazu
Jun 22, 2012·The New England Journal of Medicine·Edouard Vannier, Peter J Krause
Jul 27, 2012·Nucleic Acids Research·Emmanuel CornillotChoukri Ben Mamoun
Aug 30, 2012·Biochemistry·Arden PerkinsP Andrew Karplus
Jun 12, 2013·Annual Review of Biochemistry·Yujin E KimF Ulrich Hartl
Jul 6, 2013·Journal of Experimental Botany·Janine KönigKarl-Josef Dietz
Sep 13, 2013·The Journal of Veterinary Medical Science·Tatsunori MasataniXuenan Xuan
Aug 6, 2014·Parasitology Research·Hassan HakimiShin-ichiro Kawazu
Dec 3, 2014·Free Radical Biology & Medicine·P Andrew Karplus
Feb 28, 2015·BioMed Research International·Soraya S BoschCarsten Wrenger
Jun 13, 2015·Trends in Biochemical Sciences·Arden PerkinsP Andrew Karplus
Jan 27, 2016·Molecules and Cells·Mark B Hampton, Karina M O'Connor
Jan 28, 2016·Molecules and Cells·Bernard KnoopsOksana Kuznetsova

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Citations

Nov 11, 2021·Microbiology Spectrum·Ezinne AjaHansel M Fletcher

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Methods Mentioned

BETA
PCR
electrophoresis
Cytomation
Reverse Transcription
PCRs
covalent folding

Software Mentioned

Genetyx
microti
Basic Local Alignment Search Tool ( BLAST )
BLAST
SignalP
GraphPad PRISM

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