A novel three-dimensional culture system allows prolonged culture of functional human granulosa cells and mimics the ovarian environment

Tissue Engineering. Part a
Katarzyna Kossowska-TomaszczukArnaud Scherberich

Abstract

The development of techniques allowing the growth of primordial follicles to mature follicles in vitro has much potential for both reproductive medicine and developmental research. However, human primordial and preantral follicles fail to grow after isolation from the surrounding ovarian stroma. Granulosa cells, which normally undergo apoptosis after ovulation, contain a subpopulation of ovarian follicular cells remaining viable in vitro over prolonged periods when cultured in the presence of leukemia-inhibiting factor. However, when cultured as monolayers, they progressively lose all their characteristics, such as follicle-stimulating hormone receptor and cytochrome P450-aromatase. Here, we describe a three-dimensional culture system containing type I collagen, which, together with leukemia-inhibiting factor, allowed the survival and growth of a subpopulation of granulosa cells isolated from mature ovarian follicles and supported them to proliferate into spherical structures exhibiting steroidogenic capacity, as demonstrated by P450-aromatase and 3beta-hydroxysteroid dehydrogenase. After transplantation into the ovaries of immunodeficient mice, these cells became localized preferentially within antral follicles and the prolong...Continue Reading

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A Ben-Ze'ev, A Amsterdam
May 1, 1984·Biology of Reproduction·L F FanjulA J Hsueh
Jan 1, 1994·Journal of Reproduction and Fertility·N I Boland, R G Gosden
Oct 1, 1995·Human Reproduction·B Abdel-RahmanE Pergament
May 15, 1997·Cancer·B E MillerD M Gershenson
Oct 2, 1998·Human Reproduction·D K Gardner, M Lane
Nov 26, 1998·Biology of Reproduction·H F RodgersR J Rodgers
Jun 26, 1999·Cell and Tissue Research·I L van WezelR J Rodgers
Jul 20, 1999·Molecular and Cellular Endocrinology·R J RodgersH F Irving-Rodgers
Feb 13, 2001·Fertility and Sterility·R AbirZ Ben Rafael
Feb 13, 2001·Molecular and Cellular Endocrinology·R J RodgersT C Lavranos
Sep 19, 2001·Genetics·A M Roy-EngelP L Deininger
May 29, 2003·Molecular and Cellular Endocrinology·Hitoshi OkamuraTakashi Ohba
Feb 24, 2005·Stem Cells and Development·Boon Chin HengSoon Chye Ng
Sep 15, 2005·Cell and Tissue Research·Helen F Irving-Rodgers, Raymond J Rodgers
Oct 6, 2005·Journal of Cellular Physiology·Jörn BullwinkelThomas Scholzen
Sep 1, 2006·Seminars in Reproductive Medicine·Courtney B BerkholtzTeresa K Woodruff
Feb 19, 2009·Stem Cells·Katarzyna Kossowska-TomaszczukHong Zhang

❮ Previous
Next ❯

Citations

Jul 31, 2013·Journal of Assisted Reproduction and Genetics·Edo DzaficIrma Virant-Klun
Mar 14, 2013·BioMed Research International·Katarzyna Kossowska-Tomaszczuk, Christian De Geyter
Dec 14, 2011·Molecular Carcinogenesis·Lauren C KimlinVictoria M Virador
Aug 19, 2014·Reproductive Biomedicine Online·Federica RivaAndrea Casasco
Nov 21, 2014·Biology of Reproduction·Jeffrey T ThornePhyllis C Leppert
Mar 16, 2017·Journal of Cellular Physiology·Monica YadavSuneel K Onteru
May 8, 2019·Journal of Tissue Engineering and Regenerative Medicine·Eun ChoSeung-Yup Ku
Jan 9, 2020·Reproduction in Domestic Animals = Zuchthygiene·Martina ColomboGaia Cecilia Luvoni
Nov 12, 2016·Biomedical Materials·Sunyoung JooAnthony Atala
Feb 2, 2017·Reproduction, Fertility, and Development·Assiel J YounisRonit Abir
Oct 20, 2016·Tissue Engineering and Regenerative Medicine·Amin TamadonSeung-Yup Ku

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
FACS

Software Mentioned

Rapid Scan

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis