A novel transcriptional network for the androgen receptor in human epididymis epithelial cells

Molecular Human Reproduction
Rui YangAnn Harris

Abstract

What is the transcriptional network governed by the androgen receptor (AR) in human epididymis epithelial (HEE) cells from the caput region and if the network is tissue-specific, how is this achieved? About 200 genes are differentially expressed in the caput HEE cells after AR activation; the AR transcriptional network is tissue-specific and may be mediated in part by distinct AR co-factors including CAAT-enhancer binding protein beta (CEBPB) and runt-related transcription factor 1 (RUNX1). Little is known about the AR transcriptional program genome wide in HEE cells, nor its co-factors in those cells. AR has been best studied in the prostate gland epithelium and prostate cancer cell lines, due to the important role of this factor in prostate cancer. However AR-associated differentially expressed genes (DEGs) and AR co-factors have not yet been compared between human epididymis and prostate epithelial cells. Caput HEE cells from two donors were exposed to the synthetic androgen R1881 at 1 nM for 12-16 h after 72 h of hormone starvation. Chromatin was prepared from R1881-treated and vehicle control HEE cells. AR-associated chromatin was purified by chromatin immunoprecipitation (ChIP) and AR occupancy genome wide was revealed by...Continue Reading

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Citations

Mar 14, 2019·Endocrine-related Cancer·Suzan StellooWilbert Zwart
May 3, 2019·Andrology·J A BrowneA Harris
Jul 22, 2020·Journal of Cellular and Molecular Medicine·Jenny L KerschnerAnn Harris
Jan 3, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jenny L KerschnerAnn Harris
Jan 12, 2021·Saudi Journal of Biological Sciences·Yasmine Asaad MansourGehad A H El-Bargeesy
Mar 9, 2021·The FEBS Journal·Hannah AsheDaniel Gioeli

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