PMID: 1762919Dec 25, 1991

A novel translation initiation region from Mycoplasma genitalium that functions in Escherichia coli

Nucleic Acids Research
S LoechelP C Hu

Abstract

The tuf gene of Mycoplasma genitalium uses a signal other than a Shine-Dalgarno sequence to promote translation initiation. We have inserted the translation initiation region of this gene in front of the Escherichia coli lacZ gene and shown that it is recognized by the translational machinery of E. coli; the signal operates in vivo at roughly the same efficiency as a synthetic Shine-Dalgarno sequence. The M. genitalium sequence was also used to replace the native translation initiation region of the cat gene. When assayed in E. coli, the M. genitalium sequence is equivalent to a Shine-Dalgarno sequence in stimulating translation of this mRNA also. Site-directed mutagenesis enabled us to identify some of the bases that comprise the functional sequence. We propose that the sequence UUAACAACAU functions as a ribosome binding site by annealing to nucleotides 1082-1093 of the E. coli 16S rRNA. The activity of this sequence is enhanced when it is present in the loop of a stem-and-loop structure. Additional sequences both upstream and downstream of the initiation codon are also involved, but their role has not been elucidated.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 11, 1989·Nucleic Acids Research·J M InamineP C Hu
Dec 11, 1989·Nucleic Acids Research·S LoechelP C Hu
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D R Gallie, C I Kado
Jan 1, 1988·Annual Review of Biochemistry·L Gold
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno

Citations

Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·Jayati SenguptaJ Frank
Apr 3, 2007·Journal of Bacteriology·India G Hook-BarnardMark A McIntosh
Aug 18, 2006·Molekuliarnaia biologiia·I V Boni
Jan 1, 1996·Annual Review of Microbiology·K Dybvig, L L Voelker
Mar 10, 2000·Biochemical and Biophysical Research Communications·A GolshaniIvan G Ivanov
Jun 30, 2000·Biochemical and Biophysical Research Communications·A GolshaniIvan G Ivanov
Jun 3, 2009·Molecular Microbiology·Hervé JacquierBéatrice Berçot
Jul 18, 1997·Biochemical and Biophysical Research Communications·A GolshaniM G AbouHaidar
Aug 10, 2013·Journal of the American Chemical Society·Shuntaro TakahashiYoshio Okahata
Jan 2, 2021·Frontiers in Microbiology·Marie-Claude CarrierEric Massé

Related Concepts

Lactogest
DNA Mutational Analysis
DNA Helix Destabilizing Proteins
Alkalescens-Dispar Group
Yeast Proteins
Mycoplasma putrefaciens
Nucleolar Proteins
RNA Conformation
Regulatory Sequences, Nucleic Acid
Ribosomes

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