A novel transplantable model of lung cancer-associated tissue loss and disrupted muscle regeneration.

Skeletal Muscle
Paige C Arneson-WissinkJason D Doles

Abstract

Cancer-associated muscle wasting (CAW), a symptom of cancer cachexia, is associated with approximately 20% of lung cancer deaths and remains poorly characterized on a mechanistic level. Current animal models for lung cancer-associated cachexia are limited in that they (1) primarily employ flank transplantation methods, (2) have short survival times not reflective of the patient condition, and (3) are typically performed in young mice not representative of mean patient age. This study investigates a new model for lung cancer-associated cachexia that can address these issues and also implicates muscle regeneration as a contributor to CAW. We used tail vein injection as a method to introduce tumor cells that seed primarily in the lungs of mice. Body composition of tumor-bearing mice was longitudinally tracked using NMR-based, echo magnetic resonance imaging (echoMRI). These data were combined with histological and molecular assessments of skeletal muscle to provide a complete analysis of muscle wasting. In this new lung CAW model, we observed (1) progressive loss in whole body weight, (2) progressive loss of lean and fat mass, (3) a circulating cytokine/inflammatory profile similar to that seen in other models of CAW, (4) histolog...Continue Reading

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Citations

Apr 15, 2020·Functional & Integrative Genomics·Maria Malane Magalhães MunizLucia Galvão de Albuquerque
Aug 25, 2021·Experimental Cell Research·Paige C Arneson-Wissink, Jason D Doles

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Methods Mentioned

BETA
RNAseq
PCA
PCR

Software Mentioned

GraphPad Prism
TIBCO Spotfire
MyoVision
IDEXX
ImageJ

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