A Novel Tree Shrew (Tupaia belangeri) Model of Glaucoma.

Investigative Ophthalmology & Visual Science
Brian C SamuelsChristopher A Girkin

Abstract

Primates and rodents are used widely as animal models of glaucoma, but each has significant limitations. Researchers need additional animal models that closely resemble the relevant anatomy and pathologic features of the human disease to more quickly advance research. We validate a novel glaucoma animal model in tree shrews (Tupaia belangeri). Experimental glaucoma was induced in adult tree shrews (n = 8) by injecting 50 μL of a 25 mg/mL ferromagnetic bead solution into the anterior chamber. Beads were directed into the iridocorneal angle with a magnet to impede aqueous outflow. Animals were followed for 3 months with weekly IOP measurements and biweekly spectral domain optical coherence tomography (SD-OCT) images of the optic nerve head. Histopathology of the optic nerve and optic nerve axon counts were completed at the end of the study. The 12-week average mean IOP was 22.7 ± 3.6 and 8.6 ± 2.9 mm Hg in the treated and control eyes, respectively. Longitudinal analysis showed significant retinal nerve fiber layer (RNFL) thinning throughout the study. Axon counts were significantly reduced (59.7%) in treated versus control eyes. SD-OCT imaging showed cupping and posterior displacement of the lamina cribrosa in glaucomatous eyes....Continue Reading

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Citations

Mar 11, 2020·Current Eye Research·Alan LeJoseph L Demer
Apr 24, 2020·Molecular Medicine Reports·Yueqi BaoYu Song
Aug 11, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Pulin CheQiang Ding
May 18, 2020·Scientific Reports·Matthew D RitchC Ross Ethier
Aug 15, 2019·Progress in Retinal and Eye Research·Craig BooteMichael J A Girard
Jan 30, 2021·Translational Vision Science & Technology·James R TribblePete A Williams
Mar 27, 2020·Current Opinion in Biomedical Engineering·Rafael GrytzIan A Sigal
Jan 19, 2022·Investigative Ophthalmology & Visual Science·Jessica V JasienBrian C Samuels

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Methods Mentioned

BETA
imaging technique

Software Mentioned

CellSens
Fiji ( ImageJ Raw
AxonMaster

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